A micro satellite-based genetic linkage map of the waterflea, Daphnia pulex:: On the prospect of crustacean genomics

被引:66
作者
Cristescu, Melania E. A. [1 ]
Colbourne, John K.
Radivojc, Jelena
Lynch, Michael
机构
[1] Indiana Univ, Dept Biol, Bloomington, IN 47405 USA
[2] Indiana Univ, Ctr Genom & Bioinformat, Bloomington, IN 47405 USA
基金
美国国家科学基金会; 加拿大自然科学与工程研究理事会;
关键词
Crustacea; transmission ratio distortion; microsatellite DNA; recombination; genetic crosses; comparative genomics; Daphnia; linkage;
D O I
10.1016/j.ygeno.2006.03.007
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
We describe the first genetic linkage map for Daphnia pulex using 185 microsatellite markers, including 115 new markers reported in this study. Our approach was to study the segregation of polymorphisms in 129 F-2 progeny of one F-1 hybrid obtained by crossing two genetically divergent lineages of Daphnia isolated from two Oregon populations. The map spanned 1206 Kosambi cM and had an average intermarker distance of 7 cM. Linkage groups ranged in size from 7 to 185 cM and contained 4 to 27 markers. The map revealed 12 linkage groups corresponding to the expected number of chromosomes and covers approximately 87% of the genome. Tests for random segregation of alleles at individual loci revealed that 21% of the markers showed significant transmission ratio distortion (primarily homozygote deficiency) likely due to markers being linked to deleterious recessive alleles. This map will become the anchor for the physical map of the Daphnia genome and will serve as a starting point for mapping single and quantitative trait loci affecting ecologically important phenotypes. By mapping 342 tentative orthologous gene pairs (Daphnia/Drosophila) into the Daphnia linkage map, we facilitate future comparative projects. (c) 2006 Elsevier Inc. All rights reserved.
引用
收藏
页码:415 / 430
页数:16
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