Biological response of chondrocytes to hydrogels

被引：28

作者：

Elisseeff, JH

Lee, A

Kleinman, HK

Yamada, Y

机构：

[1] Johns Hopkins Univ, Dept Biomed Engn, Whitaker Inst Biomed Engn, Baltimore, MD 21218 USA

[2] Natl Inst Dent & Craniofacial Res, Craniofacial Dev & Regenerat Branch, Bethesda, MD 20892 USA

来源：

REPARATIVE MEDICINE: GROWING TISSUES AND ORGANS | 2002年 / 961卷

关键词：

cartilage replacement; hydrogels; chondrocytes; bioscaffolds;

D O I：

10.1111/j.1749-6632.2002.tb03062.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Primary bovine chondrocytes were encapsulated in alginate and alginate combined with cartilage matrix extract, Cartrigel, for the purpose of cartilage tissue engineering. The cell constructs were incubated in vitro and gene expression of cartilage-specific extracellular matrix molecules was quantitated and localized with in situ hybridization with a decrease in expression observed in the alginate-Cartrigel constructs. Further understanding of cell response to scaffolds will allow rational design and development of hydrogels for cartilage tissue engineering.

引用

页码：118 / 122

页数：5

共 14 条

[1] Perlecan is essential for cartilage and cephalic development [J].