Enhanced introduction of gold nanoparticles into vital Acidothiobacillus ferrooxidans by carbon nanotube-based microwave electroporation

被引:95
作者
Rojas-Chapana, JA
Correa-Duarte, MA
Ren, ZF
Kempa, K
Giersig, M
机构
[1] Ctr Adv European Studies & Res, Nanoparticle Technol Div, D-53175 Bonn, Germany
[2] Boston Coll, Boston, MA USA
关键词
D O I
10.1021/nl049699n
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
A novel method was developed to electroporate gram-negative bacteria (Acidothiobacillus ferrooxidans) via multiwall carbon nanotubes (CNTs). CNTs are metallic, ultra strong nanofibers of similar to10 nm in diameter and many microns long. When placed in an electric field, the CNTs strongly enhance the electric field at their ends by a factor of 10-100, making them ideal for localized electroporation. Addition of CNTs into a solution containing bacteria and gold nanoparticles (GNPs) and subsequent exposure to microwave radiation facilitates a rapid transport of GNPs across the cell wall, without affecting the cell viability.
引用
收藏
页码:985 / 988
页数:4
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