QCM-D on mica for parallel QCM-D-AFM studies

被引:60
作者
Richter, RP [1 ]
Brisson, A [1 ]
机构
[1] Univ Bordeaux 1, CNRS, UMR 5471, IECB,Lab Imagerie Mol & Nano Bio Technol, F-33607 Pessac, France
关键词
D O I
10.1021/la049827n
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Quartz crystal microbalance with dissipation monitoring (QCM-D) has developed into a recognized method to study adsorption processes in liquid, such as the formation of supported lipid bilayers and protein adsorption. However, the large intrinsic roughness of currently used gold-coated or silica-coated QCM-D sensors limits parallel structural characterization by atomic force microscopy (AFM). We present a method for coating QCM-D sensors with thin mica sheets operating in liquid with high stability and sensitivity. We define criteria to objectively assess the reliability of the QCM-D measurements and demonstrate that the mica-coated sensors can be used to follow the formation of supported lipid membranes and subsequent protein adsorption. This method allows combining QCM-D and AFM investigations on identical supports, providing detailed physicochemical and structural characterization of model membranes.
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收藏
页码:4609 / 4613
页数:5
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