High-performance liquid chromatographic determination of quercetin and isorhamnetin in rat tissues using β-glucuronidase and acid hydrolysis

被引：25

作者：

Morice, PC ^{[1
]}

Wood, SG ^{[1
]}

Duthie, GG ^{[1
]}

机构：

[1] Rowett Res Inst, Aberdeen, Scotland

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2000年 / 738卷 / 02期

关键词：

quercetin; isorhamnetin; beta-glucuronidase;

D O I：

10.1016/S0378-4347(99)00520-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Quercetin is a plant polyphenol which is present in the diet as an aglycone and as sugar conjugates. Despite potent vasodilatory and antioxidant effects in vitro, destruction by intestinal organisms has been assumed to Limit its nutritional relevance in the rat. However, we have refined extraction techniques using P-glucuronidase followed by acid hydrolysis. Following this with HPLC methodology with post-column derivatisation, we have detected significant concentrations of quercetin and its metabolite, isorhamnetin, in tissues of rats maintained on quercetin-rich diets. Percentage recoveries are greater than 95% and intra-batch variation does not exceed 7% suggesting that the method may be useful in further studies of the biological role of this flavonoid. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：413 / 417

页数：5

共 10 条

[1] Absorption and excretion of conjugated flavonols, including quercetin-4′-O-β-glucoside and isorhamnetin-4′-O-β-glucoside by human volunteers after the consumption of onions [J].