Detection of gelatinase B activity in serum of gastric cancer patients

被引：22

作者：

Dragutinovic, Vesna V.

Radovanovic, Nebojsa S.

T Izrael-Zivkovic, Lidija

Vrvic, Miroslav M.

机构：

[1] Univ Belgrade, Sch Med, Inst Chem, Belgrade 11000, Serbia

[2] Clin Ctr Serbia, Ctr Esophagogastr Surg, Inst Digest Dis, Belgrade 11000, Serbia

[3] Univ Belgrade, Fac Chem, Belgrade 11000, Serbia

来源：

WORLD JOURNAL OF GASTROENTEROLOGY | 2006年 / 12卷 / 01期

关键词：

matrix metalloproteinase-9; gastric cancer; proteolytic activity; inhibition;

D O I：

10.3748/wjg.v12.i1.105

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

AIM: To determine the proteolytic activity and expression of gelatinase B in serum of gastric cancer patients and their correlation with the stage of the tumor. METHODS: Sera from 23 patients who underwent surgery for primary gastric cancer as the experimental group and from 11 as the control group were used to determine the proteolytic activity and its inhibition by EDTA and 1,10-phenanthroline. Gelatinase B activity was detected by SDS polyacrylamide gel electrophoresis (SDS-PAGE) and SDS-PAGE zymography. RESULTS: Proteolytic enzyme activity was increased in gastric cancer patients when compared to the control group (P<0.05). The proteinases were determined to be metalloproteinases upon inhibition test with specific metalloproteinase inhibitors 1,10-phenanthroline (P<0.05) and EDTA (P<0.01). SDS-PAGE and SDS-PAGE zymography revealed gelatinase B (proMMP-9) activity and its molecular mass of 92 ku. CONCLUSION: Proteinase activity is overexpressed in serum of gastric cancer patients. Gelatinase B in serum plays an important role in the progression of gastric cancer. ProMMP-9 can be used as a marker for invasiveness of gastric cancer. (C) 2006 The WJG Press. All rights reserved.

引用

页码：105 / 109

页数：5

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