Cryo-electron microscopy of vitreous sections of native biological cells and tissues

被引：185

作者：

Al-Amoudi, A

Norlen, LPO

Dubochet, J

机构：

[1] Univ Lausanne, Lab Anal Ultrastruct, CH-1015 Lausanne, Switzerland

[2] Univ Geneva, Dept Phys, Appl Phys Grp, CH-1211 Geneva, Switzerland

来源：

JOURNAL OF STRUCTURAL BIOLOGY | 2004年 / 148卷 / 01期

关键词：

desmosomes; cyanobacteria; high-pressure freezing; vitrification; intermediate filament; cryo-section;

D O I：

10.1016/j.jsb.2004.03.010

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Cryo-electron microscopy of vitreous sections (CEMOVIS) is, in principle, the ultimate method of specimen preparation. It consists in ultra-rapid cooling of a sizable sample of biological material that is cut into thin sections. These are subsequently observed at low temperature in their fully hydrated vitreous state. Here, we show that CEMOVIS reveals the native state of cells and tissues with unprecedented quality and resolution. What is seen differs considerably from what conventional electron microscopy has shown previously and it is seen with more details. Our findings are demonstrated with images of cyanobacteria and skin. (C) 2004 Elsevier Inc. All rights reserved.

引用

页码：131 / 135

页数：5

共 30 条

[1]

Proteomics: The society of proteins [J].