Heat Shock Factors HsfB1 and HsfB2b Are Involved in the Regulation of Pdf1.2 Expression and Pathogen Resistance in Arabidopsis

被引：131

作者：

Kumar, Mukesh

Busch, Wolfgang ^{[1
]}

Birke, Hannah ^{[2
]}

Kemmerling, Birgit ^{[3
]}

Nuernberger, Thorsten ^{[3
]}

Schoeffl, Friedrich ^{[2
]}

机构：

[1] Max Planck Inst Entwicklungsbiol, Mol Biol Abt, D-72076 Tubingen, Germany

[2] Univ Tubingen, Zentrum Mol Biol Pflanzen Allgemeine Genet, D-72076 Tubingen, Germany

[3] Univ Tubingen, Zentrum Mol Biol Pflanzen Biochem Pflanzen, D-72076 Tubingen, Germany

来源：

MOLECULAR PLANT | 2009年 / 2卷 / 01期

关键词：

Abiotic; environmental stress; transcriptional control and transcription factors; transcriptome analysis; defense responses; disease resistance; Arabidopsis; STRESS TRANSCRIPTION FACTORS; PLANT DEFENSIN GENE; SALICYLIC-ACID; ACTIVATOR FUNCTION; RESPONSE PATHWAYS; BINDING-PROTEIN; GCC-BOX; JASMONATE; IDENTIFICATION; THERMOTOLERANCE;

D O I：

10.1093/mp/ssn095

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In order to assess the functional roles of heat stress-induced class B-heat shock factors in Arabidopsis, we investigated T-DNA knockout mutants of AtHsfB1 and AtHsfB2b. Micorarray analysis of double knockout hsfB1/hsfB2b plants revealed as strong an up-regulation of the basal mRNA-levels of the defensin genes Pdf1.2a/b in mutant plants. The Pdf expression was further enhanced by jasmonic acid treatment or infection with the necrotrophic fungus Alternaria brassicicola. The single mutant hsfB2b and the double mutant hsfB1/B2b were significantly improved in disease resistance after A. brassicicola infection. There was no indication for a direct interaction of Hsf with the promoter of Pdf1.2, which is devoid of perfect HSE consensus Hsf-binding sequences. However, changes in the formation of late HsfA2-dependent HSE binding were detected in hsfB1/B2b plants. This suggests that HsfB1/B2b may interact with class A-Hsf in regulating the shut-off of the heat shock response. The identification of Pdf genes as targets of Hsf-dependent negative regulation is the first evidence for an interconnection of Hsf in the regulation of biotic and abiotic responses.

引用

页码：152 / 165

页数：14

共 44 条

[1] Tomato heat stress transcription factor HsfB1 represents a novel type of general transcription coactivator with a histone-like motif interacting with the plant CREB binding protein ortholog HAC1 [J].

Bharti, K ;

von Koskull-Döring, P ;

Bharti, S ;

Kumar, P ;

Tintschl-Körbitzer, A ;

Treuter, E ;

Nover, L .

PLANT CELL, 2004, 16 (06) :1521-1535

[2] The cpr5 mutant of Arabidopsis expresses both NPR1-dependent and NPR1-independent resistance [J].

Bowling, SA ;

Clarke, JD ;

Liu, YD ;

Klessig, DF ;

Dong, XN .

PLANT CELL, 1997, 9 (09) :1573-1584

[3]

BROEKAERT WF, 1995, PLANT PHYSIOL, V108, P1353, DOI [10.1016/j.eclinm.2021.100771, 10.1016/j.jaap.2012.10.004, 10.1016/j.envsoft.2012.10.004, 10.1016/j.coelec.2021.100721, 10.1016/j.chiabu.2021.105188, 10.1016/j.tourman.2012.10.007, 10.1016/j.biortech.2014.10.140, 10.1016/j.carres.2021.108368]

[4] A role for the GCC-box in jasmonate-mediated activation of the PDF1.2 gene of Arabidopsis [J].