A Photinus pyralis and Luciola italica Chimeric Firefly Luciferase Produces Enhanced Bioluminescence

被引：20

作者：

Branchini, Bruce R. ^{[1
]}

Southworth, Tara L. ^{[1
]}

Fontaine, Danielle M. ^{[1
]}

Davis, Audrey L. ^{[1
]}

Behney, Curran E. ^{[1
]}

Murtiashaw, Martha H. ^{[1
]}

机构：

[1] Connecticut Coll, Dept Chem, New London, CT 06320 USA

来源：

BIOCHEMISTRY | 2014年 / 53卷 / 40期

基金：

美国国家科学基金会;

关键词：

CRYSTAL-STRUCTURE;

D O I：

10.1021/bi501202u

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report the enhanced bioluminescence properties of a chimeric enzyme (PpyLit) that contains the N-domain of recombinant Photinus pyralis luciferase joined to the C-domain of recombinant Luciola italica luciferase. Compared to the P. pyralis enzyme, the novel PpyLit chimera exhibited 1.8-fold enhanced flash-height specific activity, 2.0-fold enhanced integration-based specific activity, 2.9-fold enhanced catalytic efficiency (k(cat)/K-m), and a 1.4-fold greater bioluminescence quantum yield. The results of this study provide an underlying basis of this unusual example of a chimeric enzyme with enhanced catalytic properties that are not simply the sum of the contributions of the two luciferases.

引用

页码：6287 / 6289

页数：3

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