Antioxidative activity of polysaccharide fractions isolated from Lycium barbarum Linnaeus

被引：323

作者：

Lin, C. L. ^{[2
]}

Wang, C. C. ^{[1
]}

Chang, S. C. ^{[1
]}

Inbaraj, B. Stephen ^{[1
]}

Chen, B. H. ^{[1
,3
]}

机构：

[1] Fu Jen Catholic Univ, Dept Food Sci, Taipei 242, Taiwan

[2] Cathay Gen Hosp, Dept Endocrinol & Metab, Taipei, Taiwan

[3] Fu Jen Catholic Univ, Grad Inst Med, Taipei 242, Taiwan

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2009年 / 45卷 / 02期

关键词：

Lycium barbarum Linnaeus; Polysaccharide; Antioxidative activity; CHEMICAL-COMPOSITION; TEA; IDENTIFICATION; PURIFICATION; XANTHAN;

D O I：

10.1016/j.ijbiomac.2009.04.014

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Antioxidant activity of polysaccharide fractions isolated from Lycium barbarum Linnaeus was evaluated. Polysaccharides were extracted with boiling water, followed by precipitating with ethanol, protein hydrolysis, dialysis, and fractionation with a DEAE-Sepharose CL-6B column. A total of 4 fractions, including 1 neutral polysaccharide (LBPN) and 3 acidic polysaccharides were obtained, and compared with crude polysaccharide (CP), crude extract of polysaccharide (CE), deproteinated polysaccharide (DP), and deproteinated and dialyzed polysaccharide (DDP) for antioxidative activity. With the exception of CE and DDP, most polysaccharides were effective in scavenging DPPH and ABTS(center dot)* free radicals, superoxide anion and hydroxyl radical at 1000 mu g/mL. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：146 / 151

页数：6

共 30 条

[1]

Chemical, molecular, and structural characterization of alkali extractable nonstarch polysaccharides from Job's tears [J].

Apirattananusorn, Supaporn ;

Tongta, Sunanta ;

Cui, Steve W. ;

Wang, Qi .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2008, 56 (18) :8549-8557

[2]

Asker M. M. S., 2007, Journal of Applied Sciences Research, P1170

[3]

Antioxidant activities of different fractions of polysaccharide conjugates from green tea (Camellia Sinensis) [J].

Chen, Haixia ;

Zhang, Min ;

Qu, Zhishuang ;

Xie, Bijun .

FOOD CHEMISTRY, 2008, 106 (02) :559-563

[4]

Quantification of uronic acids in tea polysaccharide conjugates and their antioxidant properties [J].

Chen, HX ;

Zhang, M ;

Xie, BJ .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2004, 52 (11) :3333-3336

[5]

Anti-diabetic effects of water extract and crude polysaccharides from tuberous root of Liriope spicata var. prolifera in mice [J].

Chen, Xianghong ;

Bai, Xue ;

Liu, Yihui ;

Tian, Luanyuan ;

Zhou, Jianqiu ;

Zhou, Qun ;

Fang, Jinbo ;

Chen, Jiachun .

JOURNAL OF ETHNOPHARMACOLOGY, 2009, 122 (02) :205-209

[6]

Chemical properties and immunostimulatory activity of a water-soluble polysaccharide from the clam of Hyriopsis cumingii Lea [J].

Dai, Zhiyuan ;

Zhang, Hong ;

Zhang, Yangping ;

Wang, Honghai .

CARBOHYDRATE POLYMERS, 2009, 77 (02) :365-369

[7]

A COLORIMETRIC METHOD FOR THE DETERMINATION OF SUGARS [J].

DUBOIS, M ;

GILLES, K ;

HAMILTON, JK ;

REBERS, PA ;

SMITH, F .

NATURE, 1951, 168 (4265) :167-167

[8]

Immunomodulation and antitumor activity by a polysaccharide-protein complex from Lycium barbarum [J].

Gan, L ;

Zhang, SH ;

Yang, XL ;

Xu, HB .

INTERNATIONAL IMMUNOPHARMACOLOGY, 2004, 4 (04) :563-569

[9]

Antioxidant activity of different phenolic fractions separated from an Italian red wine [J].

Ghiselli, A ;

Nardini, M ;

Baldi, A ;

Scaccini, C .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1998, 46 (02) :361-367

[10]

Determination of carotenoids and their esters in fruits of Lycium barbarum Linnaeus by HPLC-DAD-APCI-MS [J].

Inbaraj, B. Stephen ;

Lu, H. ;

Hung, C. F. ;

Wu, W. B. ;

Lin, C. L. ;

Chen, B. H. .

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2008, 47 (4-5) :812-818

← 1 2 3 →