An improved method for deleting large regions of Escherichia coli K-12 chromosome using a combination of Cre/loxP and λ Red

被引：21

作者：

Fukiya, S ^{[1
]}

Mizoguchi, H ^{[1
]}

Mori, H ^{[1
]}

机构：

[1] Japan Bioind Assoc, Tokyo Res Labs, Kyowa Hakko Branch, Machida, Tokyo 1948533, Japan

来源：

FEMS MICROBIOLOGY LETTERS | 2004年 / 234卷 / 02期

关键词：

Escherichia coli; genome engineering; Cre/loxP; lambda Red; large deletion;

D O I：

10.1016/j.femsle.2004.03.042

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We have established an improved large deletion method in Escherichia coli genome using a combination of two different recombination systems,. Red and CrelloxP. The loxP site could be rapidly and efficiently integrated in the genome by, Red and large deletions of both 117- and 165-kbp regions could be generated in 100% efficiency by CrelloxP. Comparative genomic hybridization microarray experiments of deletion strains indicated that deletions were generated only in expected regions of the genome. These results have demonstrated that the method is useful for genome engineering in E. coli. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：325 / 331

页数：7

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