HPLC analysis of fat-soluble vitamins on standard and narrow bore columns with UV, electrochemical and particle beam MS detection

被引：25

作者：

Andreoli, R ^{[1
]}

Careri, M ^{[1
]}

Manini, P ^{[1
]}

Mori, G ^{[1
]}

Musci, M ^{[1
]}

机构：

[1] UNIV PARMA, DIPARTIMENTO CHIM GEN & INORGAN, I-43100 PARMA, ITALY

来源：

CHROMATOGRAPHIA | 1997年 / 44卷 / 11-12期

关键词：

column liquid chromatography; UV and electrochemical detection; particle beam mass spectrometry; fat-souble vitamin; standard-bore and narrow-bore columns;

D O I：

10.1007/BF02466663

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The application of different detection techniques; UV, electrochemical (ED) and particle beam-mass spectrometry (PB-MS) for the high-performance liquid chromatography analysis of fat-soluble vitamins were studied. Separation of all compounds examined was performed using both C8 standard-bore and narrow-bore columns for UV and MS detection, whereas in the case of HPLC-ED, a C8 standard-bore column only was used. Detector responses were studied in terms of linear range, detection limit and precision. The HPLC methods with UV, PB-MS and electrochemical detection were applied to the assay of vitamins A and E in an infant-formula, powdered-milk sample. Recovery studies showed good results both for vitamins A and E, within-run precision being 2-4 % for LC-UV and LC-ED techniques. In addition, excellent agreement between the results obtained with the detection systems explored was found.

引用

页码：605 / 612

页数：8

共 15 条

[1] ELECTROCHEMICAL DETECTION OF RETINOIDS USING NORMAL PHASE HPLC [J].