Monitoring the secretion from single cells with temporal and spatial resolution

被引：4

作者：

Huang, WH ^{[1
]}

Hu, S ^{[1
]}

Pang, DW ^{[1
]}

Wang, ZL ^{[1
]}

Cheng, JK ^{[1
]}

机构：

[1] Wuhan Univ, Analyt Sci Ctr, Dept Chem & Environm Sci, Wuhan 430072, Peoples R China

来源：

CHINESE SCIENCE BULLETIN | 2000年 / 45卷 / 04期

关键词：

single cell secretion; neurotransmitters; exocytosis; electrochemical monitoring; fluorescent method;

D O I：

10.1007/BF02909756

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The secretion from cells plays a significant role in life activities and in the fields of neurobiology, cell biology, clinic, pathology, pharmacology etc. Thus to investigate the secretion at the single cell level with the developing methods is a frontier cross-discipline research activity. The contents, methods and new progress of this research have been reviewed by electrochemical monitoring combined with patch-clamp techniques, fluorescent detection and immunoassay, and the prospects on the frontier of the research area have been discussed.

引用

页码：289 / 295

页数：7

共 37 条

[1] Effects of intravesicular H+ and extracellular H+ and Zn2+ on insulin secretion in pancreatic beta cells [J].

Aspinwall, CA ;

Brooks, SA ;

Kennedy, RT ;

Lakey, JRT .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (50) :31308-31314

[2] Isoproterenol evokes extracellular Ca2+ spikes due to secretory events in salivary gland cells [J].

Belan, P ;

Gardner, J ;

Gerasimenko, O ;

Gerasimenko, J ;

Mills, CL ;

Petersen, OH ;

Tepikin, AV .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (07) :4106-4111

[3] Effects of external osmotic pressure on vesicular secretion from bovine adrenal medullary cells [J].

Borges, R ;

Travis, ER ;

Hochstetler, SE ;

Wightman, RM .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (13) :8325-8331

[4] Single cell measurements of purine release using a micromachined electroanalytical sensor [J].

Bratten, CDT ;

Cobbold, PH ;

Cooper, JM .

ANALYTICAL CHEMISTRY, 1998, 70 (06) :1164-1170

[5] THE CONTROL OF CALCIUM-RELEASE IN HEART-MUSCLE [J].

CANNELL, MB ;

CHENG, H ;

LEDERER, WJ .

SCIENCE, 1995, 268 (5213) :1045-1049

[6] AMPEROMETRIC MONITORING OF STIMULATED CATECHOLAMINE RELEASE FROM RAT PHEOCHROMOCYTOMA (PC12) CELLS AT THE ZEPTOMOLE LEVEL [J].

CHEN, TK ;

LUO, GO ;

EWING, AG .

ANALYTICAL CHEMISTRY, 1994, 66 (19) :3031-3035

[7]

Cheng JK, 1997, CHEM J CHINESE U, V18, P1046

[8] Injection of ultrasmall samples and single molecules into tapered capillaries [J].

Chiu, DT ;

Hsiao, A ;

Gaggar, A ;

GarzaLopez, RA ;

Orwar, O ;

Zare, RN .

ANALYTICAL CHEMISTRY, 1997, 69 (10) :1801-1807

[9] Chemical transformations in individual ultrasmall biomimetic containers [J].

Chiu, DT ;

Wilson, CF ;

Ryttsén, F ;

Strömberg, A ;

Farre, C ;

Karlsson, A ;

Nordholm, S ;

Gaggar, A ;

Modi, BP ;

Moscho, A ;

Garza-López, RA ;

Orwar, O ;

Zare, RN .

SCIENCE, 1999, 283 (5409) :1892-1895

[10] DELAY IN VESICLE FUSION REVEALED BY ELECTROCHEMICAL MONITORING OF SINGLE SECRETORY EVENTS IN ADRENAL CHROMAFFIN CELLS [J].

CHOW, RH ;

VONRUDEN, L ;

NEHER, E .

NATURE, 1992, 356 (6364) :60-63

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