The induction of surface β-amyloid binding proteins and enhanced cytotoxicity in cultured PC-12 and IMR-32 cells by advanced glycation end products

During aging the non-enzymatic glycation of proteins and other molecules increases significantly, leading to the accumulation of advanced glycation end-products (AGEs). These AGEs enhance inflammatory and autoimmune reactions with resultant cytotoxicity. We noted in an earlier study that individuals with Alzheimer's disease exhibit enhanced expression of the receptor for advanced glycation end-products (RAGE) on the surface of their leukocytes. In order to better understand the relationship between AGEs and the cell surface binding of amyloid-beta protein (A beta) 42 we studied the effect of two AGEs: glycated bovine serum albumin (BSA), and epsilon-carboxymethyllysine-BSA (CML), a glycoxidation product, on the binding of A beta 42 to rat PC-12 and IMR-32 cells. We measured the expression of three potential cell surface receptors binding A beta 42: RAGE, P-amyloid precursor protein (beta-APP), and the alpha 7 subtype of the nicotinic acetylcholine receptor (alpha 7nAChR) by using specific antibody probes. Incubation of PC-12 or IMR-32 cells with bovine serum albumin-advanced glycation end-product (BSA-AGE) or with CML induced small but significant concentration-dependent increases in the expression of beta-APP, RAGE, and alpha 7nAChRs as measured by flow cytometry or by ELISA. Incubation of the cells with 48 mu M of either of the AGEs combined with varying concentrations (138-1100 nM) of A beta 42 resulted in the enhanced binding of the A beta 42 to the cell surface as compared with cells not exposed to the AGE co-treatment. The combination of AGE and A beta treatment also resulted in the heightened expression of all three potential A beta binding sites as well as their gene precursors. Exposure of cells to the same regimen of AGE plus A beta resulted in the production of reactive oxygen species and mitochondrial toxicity. These results are consistent with the ability of AGEs to enhance the cell surface expression of diverse A beta 42 binding sites, a factor that can lead to the enhanced binding of amyloid and subsequent cell death. (c) 2006 IBRO. Published by Elsevier Ltd. All rights reserved.