The role of antioxidants in the prevention of t-butyl hydroperoxide-induced chemiluminescence

被引：7

作者：

Gumuslu, S

Yucel, G

Aydin, M

Yesilkaya, A

Demir, AY

Aksu, TA

机构：

[1] Department of Biochemistry, Medical Faculty, Akdeniz University

来源：

INTERNATIONAL JOURNAL OF CLINICAL & LABORATORY RESEARCH | 1996年 / 26卷 / 02期

关键词：

chemiluminescence; t-butyl hydroperoxide; ascorbic acid; glutathione; uric acid; taurine;

D O I：

10.1007/BF02592354

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

An experimental system which assesses the antioxidant potential of ascorbic acid, glutathione, uric acid, and taurine was developed. The system comprised hemoglobin, luminol, t-butyl hydroperoxide, and differ ent concentrations of antioxidants in TRIS-HCl buffer (pH 7.4). Control assays were per-formed by excluding antioxidants. Chemiluminescence was detected using a liquid scintillation counter in single photon mode, All antioxidants, when applied in the appropriate concentrations, decreased the maximum chemiluminescence values. The minimum concentrations which decreased the chemiluminescence values were defined for each of the antioxidants.

引用

页码：119 / 123

页数：5

共 16 条

[1] URIC-ACID PROVIDES AN ANTIOXIDANT DEFENSE IN HUMANS AGAINST OXIDANT-CAUSED AND RADICAL-CAUSED AGING AND CANCER - A HYPOTHESIS [J].