Photoreactivation compensates for UV damage and restores infectivity to natural marine virus communities

被引:80
作者
Weinbauer, MG
Wilhelm, SW
Suttle, CA
Garza, DR
机构
[1] UNIV BRITISH COLUMBIA, DEPT EARTH & OCEAN SCI OCEANOG, VANCOUVER, BC V6T 1Z4, CANADA
[2] UNIV TEXAS, INST MARINE SCI, PORT ARANSAS, TX 78373 USA
关键词
D O I
10.1128/AEM.63.6.2200-2205.1997
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
We investigated the potential for photoreactivation to restore infectivity to sunlight-damaged natural viral communities in offshore (chlorophyll a, <0.1 mu g liter(-1)), coastal (chlorophyll a, ca. 0.2 mu g liter(-1)), and estuarine (chlorophyll a, ca. 1 to 5 pg liter(-1)) waters of the Gulf of Mexico. In 67% of samples, the light-dependent repair mechanisms of the bacterium Vibrio natriegens restored infectivity to natural viral communities which could not be repaired by light-independent mechanisms. Similarly, exposure of sunlight-damaged natural viral communities to >312-nm-wavelength sunlight in the presence of the natural bacterial communities restored infectivity to 21 to 26% of sunlight-damaged viruses in oceanic waters and 41 to 52% of the damaged viruses in coastal and estuarine waters. Wavelengths between 370 and 550 nm were responsible for restoring infectivity to the damaged viruses. These results indicate that light-dependent repair, probably photoreactivation, compensated for a large fraction of sunlight-induced DNA damage in natural viral communities and is potentially essential for the maintenance of high concentrations of viruses in surface waters.
引用
收藏
页码:2200 / 2205
页数:6
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