Tetrodotoxin detection and species identification of pufferfish in retail roasted fish fillet by DNA barcoding in China

被引：11

作者：

Li, Nan ^{[1
]}

Shen, Qing ^{[2
]}

Wang, Jiahui ^{[1
]}

Han, Chunhui ^{[1
]}

Ji, Rong ^{[1
]}

Li, Fengqin ^{[1
]}

Jiang, Tao ^{[1
]}

机构：

[1] China Natl Ctr Food Safety Risk Assessment, Minist Hlth, Key Lab Food Safety Risk Assessment, Beijing, Peoples R China

[2] Qingdao Univ, Coll Med, Dept Epidemiol & Hlth Stat, Qingdao 266071, Peoples R China

来源：

FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT | 2015年 / 32卷 / 12期

基金：

中国国家自然科学基金;

关键词：

roasted fish fillet; pufferfish; DNA barcode; Enzyme-linked immunosorbent assay (ELISA); tetrodotoxin (TTX);

D O I：

10.1080/19440049.2015.1087056

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

This study identifies the pufferfish species and detects tetrodotoxin (TTX) in roasted fish fillet samples collected in Beijing, Qingdao and Xiamen, China. The cytochrome c oxidase I (COI) gene was used as the target gene for identification of the pufferfish species in the samples. Enzyme-linked immunosorbent assay (ELISA) screened the TTX levels in samples that had been detected as containing pufferfish by DNA barcode. A total of 125 samples were identified by DNA barcodes; 32 (26%) samples contained pufferfish composition and, among them, 26 (81%) were the highly toxic species Lagocephalus lunaris. All 32 samples containing the pufferfish composition were positive for TTX with levels ranging from 100 to 63800ngg(-1). Most of the 32 samples contained the highly toxic L. lunaris. Based on the results, we suggest that the monitoring of roasted fish fillet should be strengthened and the processing procedures should be standardised to minimise TTX poisoning caused by pufferfish.

引用

页码：2148 / 2153

页数：6

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