Assessment of 123I-FIAU imaging of herpes simplex viral gene expression in the treatment of glioma

被引:27
作者
Dempsey, Mary F.
Wyper, David
Owens, Jonathan
Pimlott, Sally
Papanastassiou, Vakis
Patterson, James
Hadley, Donald M.
Nicol, Alice
Rampling, Roy
Brown, S. M.
机构
[1] Inst Neurol Sci, Glasgow, Lanark, Scotland
[2] W Scotland Radionuclide Dispensary, Glasgow, Lanark, Scotland
[3] Beatson Oncol Ctr, Glasgow, Lanark, Scotland
[4] S Moira Brown Crusade Labs Glasgow, Glasgow, Lanark, Scotland
关键词
glioma; herpes simplex virus; FIAU; molecular imaging;
D O I
10.1097/00006231-200608000-00003
中图分类号
R8 [特种医学]; R445 [影像诊断学];
学科分类号
1002 ; 100207 ; 1009 ;
摘要
Background Herpes simplex virus 1716 (HSV1716), a selectively replication competent mutant of HSV1, is under investigation as an oncolytic viral therapy in human malignant glioma. As with similar therapies, a technique for measurement of viral replication and distribution over time following virus administration is required. Imaging expression of the HSV-thymidine kinase (HSV-tk) gene offers an opportunity for non-invasive assessment of viral distribution in living subjects. This is the first study to explore the use of HSV-tk as a reporter gene and radiolabelled thymidine analogue 5-[I-123] iodo-1-(2-deoxy-2-fluoro-beta-D-arabinofuranosyl) uracil (I-123-FIAU) as a marker substrate to non-invasively monitor HSV1716 replication in humans during treatment of high-grade glioma. Methods I-123-FIAU brain SPECT imaging was undertaken in eight patients receiving intra-tumoural injection of HSV1716, before and after administration of the virus. Baseline images were acquired 3 days prior to virus administration and between 1 and 5 days following virus administration. Region of interest analysis was used to investigate whether there was an increase in I-123-FIAU concentration following virus administration due to HSV-tk expression. Result Increased I-123-FIAU accumulation due to HSV-tk expression was not detected in this study. The possible explanations for this finding are explored and design options for future studies are discussed.
引用
收藏
页码:611 / 617
页数:7
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