Aspirin inhibits serine phosphorylation of IRS-1 in muscle and adipose tissue of septic rats

被引：12

作者：

Barreiro, GC ^{[1
]}

Prattali, RR ^{[1
]}

Caliseo, CT ^{[1
]}

Fugiwara, FY ^{[1
]}

Ueno, M ^{[1
]}

Prada, PO ^{[1
]}

Velloso, LA ^{[1
]}

Saad, MJA ^{[1
]}

Carvalheira, JBC ^{[1
]}

机构：

[1] Univ Estadual Campinas, Dept Clin Med, Campinas, SP, Brazil

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2004年 / 320卷 / 03期

基金：

巴西圣保罗研究基金会;

关键词：

insulin; tyrosine phosphorylation; mitogen-activated protein kinase; sepsis;

D O I：

10.1016/j.bbrc.2004.06.048

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Whole body insulin resistance has been demonstrated in septic patients and in infected animals. In this study, we demonstrate that sepsis induces insulin resistance and that pretreatment with aspirin inhibits sepsis-induced insulin resistance. Sepsis was observed to lead to serine phosphorylation of IRS-1, a phenomenon which was reversed by aspirin in muscle and WAT, in parallel with a reduction in JNK activity. In addition, our data show an impairment of insulin activation of IR and IRS-1 tyrosine phosphorylation in septic rats and, consistent with the reduction of IRS-1 serine phosphorylation observed in septic animals pretreated with aspirin, there was an increase in IRS-1 protein levels and tyrosine phosphorylation in muscle and WAT. Overall, these results provide important new insights into the mechanism of sepsis-induced insulin resistance. (C) 2004 Elsevier Inc. All rights reserved.

引用

页码：992 / 997

页数：6

共 43 条

[1] Phosphorylation of Ser307 in insulin receptor substrate-1 blocks interactions with the insulin receptor and inhibits insulin action [J].

Aguirre, V ;

Werner, ED ;

Giraud, J ;

Lee, YH ;

Shoelson, SE ;

White, MF .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (02) :1531-1537

[2] The c-Jun NH2-terminal kinase promotes insulin resistance during association with insulin receptor substrate-1 and phosphorylation of Ser307 [J].

Aguirre, V ;

Uchida, T ;

Yenush, L ;

Davis, R ;

White, MF .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (12) :9047-9054

[3] ESTIMATES OF INVIVO INSULIN ACTION IN MAN - COMPARISON OF INSULIN TOLERANCE-TESTS WITH EUGLYCEMIC AND HYPERGLYCEMIC GLUCOSE CLAMP STUDIES [J].

BONORA, E ;

MOGHETTI, P ;

ZANCANARO, C ;

CIGOLINI, M ;

QUERENA, M ;

CACCIATORI, V ;

CORGNATI, A ;

MUGGEO, M .

JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 1989, 68 (02) :374-378

[4]

Davis RJ, 1999, BIOCHEM SOC SYMP, P1

[5] JNK1 - A PROTEIN-KINASE STIMULATED BY UV-LIGHT AND HA-RAS THAT BINDS AND PHOSPHORYLATES THE C-JUN ACTIVATION DOMAIN [J].

DERIJARD, B ;

HIBI, M ;

WU, IH ;

BARRETT, T ;

SU, B ;

DENG, TL ;

KARIN, M ;

DAVIS, RJ .

CELL, 1994, 76 (06) :1025-1037

[6] Phosphorylation of insulin receptor substrate 1 by glycogen synthase kinase 3 impairs insulin action [J].

EldarFinkelman, H ;

Krebs, EG .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (18) :9660-9664

[7]

FOLLI F, 1992, J BIOL CHEM, V267, P22171

[8] Aspirin inhibits serine phosphorylation of insulin receptor substrate 1 in tumor necrosis factor-treated cells through targeting multiple serine kinases [J].

Gao, ZG ;

Zuberi, A ;

Quon, MJ ;

Dong, ZG ;

Ye, JP .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (27) :24944-24950

[9] ROLE OF COUNTERREGULATORY HORMONES IN THE CATABOLIC RESPONSE TO STRESS [J].

GELFAND, RA ;

MATTHEWS, DE ;

BIER, DM ;

SHERWIN, RS .

JOURNAL OF CLINICAL INVESTIGATION, 1984, 74 (06) :2238-2248

[10] Modulation of insulin-stimulated degradation of human insulin receptor substrate-1 by serine 312 phosphorylation [J].

Greene, MW ;

Sakaue, H ;

Wang, LH ;

Alessi, DR ;

Roth, RA .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (10) :8199-8211

← 1 2 3 4 5 →