Positive-contrast imaging in the rabbit hind-limb of transplanted cells bearing endocytosed superparamagnetic beads

被引：16

作者：

Foltz, Warren D.

Cunningham, Charles H.

Mutsaers, Anthony J.

Conolly, Steven M.

Stewart, Duncan J.

Dick, Alexander J.

机构：

[1] St Michaels Hosp, Dept Cardiol, Toronto, ON, Canada

[2] Stanford Univ, Dept Elect Engn, Stanford, CA 94305 USA

[3] Univ Toronto, Dept Med Biophys, Toronto, ON, Canada

[4] Univ Calif Berkeley, Dept Bioengn, Berkeley, CA 94720 USA

[5] Sunnybrook & Womens Coll Hosp, Dept Cardiol, Toronto, ON, Canada

来源：

JOURNAL OF CARDIOVASCULAR MAGNETIC RESONANCE | 2006年 / 8卷 / 06期

关键词：

CMR; positive-contrast imaging; superparamagnetic beads; rabbit;

D O I：

10.1080/10976640600777819

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Positive-contrast methods can augment negative-contrast methods for CMR evaluation of transplanted cell fate in vivo. This study correlated positive-contrast signal characteristics in the rabbit hind-limb with xenograft cell number (rat fibroblasts bearing endocytosed 1 mu m Bang's particles) at different stages of Gd-DTPA administration and 1mm(2) in-plane resolution (2 minute scans, 1.5 Tesla). Linear regressions modeled satisfactorily the cell number dependencies of signal-to-noise (SNR) and area-of-enhancement (R > 0.83), over approximately a 50-fold fluctuation in cell number to a lowest detected limit of 25k cells during Gd-DTPA administration. Gd-DTPA administration elevated the slopes of parameter regressions by four or more standard deviations.

引用

收藏

页码：817 / 823

页数：7

相关论文

共 24 条

[1] Characterization of biophysical and metabolic properties of cells labeled with superparamagnetic iron oxide nanoparticles and transfection agent for cellular MR imaging [J].

Arbab, AS ;

Bashaw, LA ;

Miller, BR ;

Jordan, EK ;

Lewis, BK ;

Kalish, H ;

Frank, JA .

RADIOLOGY, 2003, 229 (03) :838-846

[2] Transplantation of progenitor cells and regeneration enhancement in acute myocardial infarction -: (TOPCARE-AMI) [J].

Assmus, B ;

Schächinger, V ;

Teupe, C ;

Britten, M ;

Lehmann, R ;

Döbert, N ;

Grünwald, F ;

Aicher, A ;

Urbich, C ;

Martin, H ;

Hoelzer, D ;

Dimmeler, S ;

Zeiher, AM .

CIRCULATION, 2002, 106 (24) :3009-3017

[3] Novel double contrast MRI technique for intramyocardial detection of percutaneously transplanted autologous cells [J].

Baklanov, DV ;

Demuinck, ED ;

Thompson, CA ;

Pearlman, JD .

MAGNETIC RESONANCE IN MEDICINE, 2004, 52 (06) :1438-1442

[4] Application of the static dephasing regime theory to superparamagnetic iron-oxide loaded cells [J].

Bowen, CV ;

Zhang, XW ;

Saab, G ;

Gareau, PJ ;

Rutt, BK .

MAGNETIC RESONANCE IN MEDICINE, 2002, 48 (01) :52-61

[5] Magnetodendrimers allow endosomal magnetic labeling and in vivo tracking of stem cells [J].

Bulte, JWM ;

Douglas, T ;

Witwer, B ;

Zhang, SC ;

Strable, E ;

Lewis, BK ;

Zywicke, H ;

Miller, B ;

van Gelderen, P ;

Moskowitz, BM ;

Duncan, ID ;

Frank, JA .

NATURE BIOTECHNOLOGY, 2001, 19 (12) :1141-1147

[6] Cell-based gene transfer to the pulmonary vasculature - Endothelial nitric oxide synthase overexpression inhibits monocrotaline-induced pulmonary hypertension [J].

Campbell, AIM ;

Kuliszewski, MA ;

Stewart, DJ .

AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY, 1999, 21 (05) :567-575

[7] POsitive contrast magnetic resonance imaging of cells labeled with magnetic nanoparticles [J].

Cunningham, CH ;

Arai, T ;

Yang, PC ;

McConnell, MV ;

Pauly, JM ;

Conolly, SM .

MAGNETIC RESONANCE IN MEDICINE, 2005, 53 (05) :999-1005

[8] Magnetic resonance fluoroscopy allows targeted delivery of mesenchymal stem cells to infarct borders in swine [J].

Dick, AJ ;

Guttman, MA ;

Raman, VK ;

Peters, DC ;

Pessanha, BSS ;

Hill, JM ;

Smith, S ;

Scott, G ;

McVeigh, ER ;

Lederman, RJ .

CIRCULATION, 2003, 108 (23) :2899-2904

[9] Detection threshold of single SPIO-Labeled cells with FIESTA [J].

Heyn, C ;

Bowen, CV ;

Rutt, BK ;

Foster, PJ .

MAGNETIC RESONANCE IN MEDICINE, 2005, 53 (02) :312-320

[10] Serial cardiac magnetic resonance imaging of injected mesenchymal stem cells [J].

Hill, JM ;

Dick, AJ ;

Raman, VK ;

Thompson, RB ;

Yu, ZX ;

Hinds, KA ;

Pessanha, BSS ;

Guttman, MA ;

Varney, TR ;

Martin, BJ ;

Dunbar, CE ;

McVeigh, ER ;

Lederman, RJ .

CIRCULATION, 2003, 108 (08) :1009-1014