Discrimination of Viable and Dead Fecal Bacteroidales Bacteria by Quantitative PCR with Propidium Monoazide

被引：166

作者：

Bae, Sungwoo ^{[1
]}

Wuertz, Stefan ^{[1
]}

机构：

[1] Univ Calif Davis, Dept Civil & Environm Engn, Davis, CA 95616 USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2009年 / 75卷 / 09期

关键词：

REAL-TIME PCR; LISTERIA-MONOCYTOGENES CELLS; ETHIDIUM MONOAZIDE; EXTRACELLULAR DNA; SOURCE TRACKING; FRESH-WATER; POLLUTION; QUANTIFICATION; SAMPLES; REMOVAL;

D O I：

10.1128/AEM.01333-08

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Propidium monoazide (PMA) was optimized to discriminate between viable and dead Bacteroides fragilis cells and extracellular DNA at different concentrations of solids using quantitative PCR. Conditions of 100 mu M PMA and a 10-min light exposure also excluded DNA from heat-treated cells of nonculturable Bacteroidales in human feces and wastewater influent and effluent.

引用

页码：2940 / 2944

页数：5

共 19 条

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