Seasonality of energetic functioning and production of reactive oxygen species by lugworm (Arenicola marina) mitochondria exposed to acute temperature changes

被引:87
作者
Keller, M
Sommer, AM
Pörtner, HO
Abele, D
机构
[1] Alfred Wegener Inst Polar & Marine Res, D-27568 Bremerhaven, Germany
[2] Int Jacobs Univ Bremen, D-28759 Bremen, Germany
关键词
lugworm; Arenicola marina; mitochondria; ROS; proton leak; metabolic regulation; temperature;
D O I
10.1242/jeb.01050
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The influence of seasonal and acute temperature changes on mitochondrial functions were studied in isolated mitochondria of the eurythermal lugworm Arenicola marina (Polychaeta), with special emphasis on the interdependence of membrane potential and radical production. Acclimatisation of lugworms to prespawning/summer conditions is associated with rising mitochondrial substrate oxidation rates, higher proton leakage rates, elevated membrane potentials, and increased production of reactive oxygen species (ROS) in isolated mitochondria, compared with mitochondria from winter animals. However, a high ROS production was compensated for by higher activities of the antioxidant enzymes catalase and superoxide dismutase, as well as lower mitochondrial densities in summer compared with winter animals. In summer animals, a higher sensitivity of the proton leakage rate to changes of membrane potential will confer better flexibility for metabolic regulation (mild uncoupling) in response to temperature change. These seasonal alterations in mitochondrial functions suggest modifications of energy metabolism in eurythermal and euryoxic organisms on intertidal mudflats during summer. In winter, low and less changeable temperatures in intertidal sedimentary environments permit higher respiratory efficiency at low aerobic metabolic rates and lower membrane potentials in A. marina mitochondria.
引用
收藏
页码:2529 / 2538
页数:10
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