Introduction of the MASH1 gene into mouse embryonic stem cells leads to differentiation of motoneuron precursors lacking Nogo receptor expression that can be applicable for transplantation to spinal cord injury

被引:43
作者
Hamada, Mari
Yoshikawa, Hideshi
Ueda, Yuji
Kurokawa, Manae S.
Watanabe, Kenji
Sakakibara, Manabu
Tadokoro, Mamoru
Akashi, Katsuya
Aoki, Haruhito
Suzuki, Noboru
机构
[1] St Marianna Univ, Sch Med, Dept Immunol & Med, Miyamae Ku, Kawasaki, Kanagawa 2168511, Japan
[2] St Marianna Univ, Sch Med, Dept Orthoped Surg, Miyamae Ku, Kawasaki, Kanagawa 2168511, Japan
[3] St Marianna Univ, Sch Med, Dept Emergency Crit Care Med, Miyamae Ku, Kawasaki, Kanagawa 2168511, Japan
[4] St Marianna Univ, Sch Med, Dept Pathol, Miyamae Ku, Kawasaki, Kanagawa 2168511, Japan
[5] St Marianna Univ, Grad Sch Med, Dept Regenerat Med, Inst Adv Med Sci, Kawasaki, Kanagawa 2168511, Japan
关键词
ES cells; differentiation; spinal cord; motoneuron; Nogo receptor; spinal cord injury; motor function;
D O I
10.1016/j.nbd.2005.12.020
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
ES cells transfected with the MASH1 gene yielded purified spinal motoneuron precursors expressing 11139 and Islet1. The cells lacked the expression of Nogo receptor that was of great advantage for axon growth after transplantation to an injured spinal cord. After transplantation, mice with the complete transection of spinal cord exhibited excellent improvement of the motor functions. Electrophysiological assessment confirmed the quantitative recovery of motor-evoked potential in the transplanted spinal cord. In the grafted spinal cord, gliosis was inhibited and Nogo receptor expression was scarcely detected. The transplanted cells labeled with GFP showed extensive outgrowth of axons positive for neurofilament middle chain, connected to each other and expressed Synaptophysin, Lim1/2 and Islet1. Thus, the in vivo differentiation into mature spinal motoneurons and the reconstitution of neuronal pathways were suggested. The grafted cell population was purified for neurons and was free from teratoma development. These therapeutic strategies may contribute to a potent treatment for spinal cord injury in future. (c) 2006 Elsevier Inc. All rights reserved.
引用
收藏
页码:509 / 522
页数:14
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