Isolation and characterization of tubular structures of cowpea mosaic virus

被引：29

作者：

Kasteel, DTJ

Wellink, J

Goldbach, RW

vanLent, JWM

机构：

[1] AGR UNIV WAGENINGEN,DEPT VIROL,NL-6709 PD WAGENINGEN,NETHERLANDS

[2] AGR UNIV WAGENINGEN,DEPT MOL BIOL,NL-6709 PD WAGENINGEN,NETHERLANDS

来源：

JOURNAL OF GENERAL VIROLOGY | 1997年 / 78卷

关键词：

D O I：

10.1099/0022-1317-78-12-3167

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Tubular structures involved in the cell-to-cell movement of cowpea mosaic virus (CPMV) were partially purified from infected cowpea protoplasts to identify the structural components. A relatively pure fraction could be obtained by differential centrifugation and this was analysed by PAGE and immunoblotting. Besides the movement protein (MP) and capsid proteins (CP) of CPMV, no other major infection-specific proteins could be detected, suggesting that host proteins are not a major structural component of the movement tubule.

引用

页码：3167 / 3170

页数：4

共 12 条

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