Inhibitory action of novel aromatic diamine compound on lipopolysaccharide-induced nuclear translocation of NF-κB without affecting IκB degradation

被引:205
作者
Shin, HM
Kim, MH
Kim, BH
Jung, SH
Kim, YS
Park, HJ
Hong, JT
Kyung, RM
Kim, Y [1 ]
机构
[1] Chungbuk Natl Univ, Coll Pharm, Cheongju 361763, South Korea
[2] Chungbuk Natl Univ, Res Ctr Bioresource & Hlth, Cheongju 361763, South Korea
[3] Chungnam Natl Univ, Coll Pharm, Taejon 305764, South Korea
[4] Seoul Natl Univ, Nat Prod Res Inst, Seoul 110460, South Korea
来源
FEBS LETTERS | 2004年 / 571卷 / 1-3期
关键词
aromatic diamine compound; NF-kappa B; I kappa B; pro-inflammatory cytokine; LPS-inducible enzyme;
D O I
10.1016/j.febslet.2004.06.056
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
4-Methyl-N'-(3-phenyl-propyl)-benzene-1,2-diamine (JSH-23) is a novel chemically synthetic compound. The aromatic diamine JSH-23 compound exhibited inhibitory effect with an IC50 value of 7.1 muM on nuclear factor (NF)-kappaB transcriptional activity in lipopolysaccharide (LPS)-stimulated macrophages; RAW 264.7, and interfered LPS-induced nuclear translocation of NF-kappaB without affecting IkappaB degradation. This mechanism of action is very rare for controlling NF-kappaB activation. Furthermore, the compound inhibited not only LPS-induced expressions of tumor necrosis factor-alpha, interleukin (IL)-1beta, IL-6 and inducible nitric oxide synthase and cyclooxygenase-2 but also LPS-induced apoptosis of the RAW 264.7 cells. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码:50 / 54
页数:5
相关论文
共 21 条
[1]   NF-kappa B: Ten years after [J].
Baeuerle, PA ;
Baltimore, D .
CELL, 1996, 87 (01) :13-20
[2]   I-KAPPA-B INTERACTS WITH THE NUCLEAR-LOCALIZATION SEQUENCES OF THE SUBUNITS OF NF-KAPPA-B - A MECHANISM FOR CYTOPLASMIC RETENTION [J].
BEG, AA ;
RUBEN, SM ;
SCHEINMAN, RI ;
HASKILL, S ;
ROSEN, CA ;
BALDWIN, AS .
GENES & DEVELOPMENT, 1992, 6 (10) :1899-1913
[3]   Bromoenol lactone promotes cell death by a mechanism involving phosphatidate phosphohydrolase-1 rather than calcium-independent phospholipase A2 [J].
Fuentes, L ;
Pérez, R ;
Nieto, ML ;
Balsinde, J ;
Balboa, MA .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (45) :44683-44690
[4]  
Hehner SP, 1999, J IMMUNOL, V163, P5617
[5]   3 NF-KAPPA-B SITES IN THE I-KAPPA-B-ALPHA PROMOTER ARE REQUIRED FOR INDUCTION OF GENE-EXPRESSION BY TNF-ALPHA [J].
ITO, CY ;
KAZANTSEV, AG ;
BALDWIN, AS .
NUCLEIC ACIDS RESEARCH, 1994, 22 (18) :3787-3792
[6]   Anti-inflammatory mode of isoflavone glycoside sophoricoside by inhibition of interleukin-6 and cyclooxygenase-2 in inflammatory response [J].
Kim, BH ;
Chung, EY ;
Ryu, JC ;
Jung, SH ;
Min, KR ;
Kim, Y .
ARCHIVES OF PHARMACAL RESEARCH, 2003, 26 (04) :306-311
[7]  
Kolenko V, 1999, J IMMUNOL, V163, P590
[8]  
LEE JH, 2003, THESIS CHUNGNAM NATL
[9]   Sophoricoside analogs as the IL-5 inhibitors from Sophora japonica [J].
Min, B ;
Oh, SR ;
Lee, HK ;
Takatsu, K ;
Chang, IM ;
Min, KR ;
Kim, Y .
PLANTA MEDICA, 1999, 65 (05) :408-412
[10]   TUMOR-NECROSIS-FACTOR ALPHA-INDUCED PHOSPHORYLATION OF I-KAPPA-B-ALPHA IS A SIGNAL FOR ITS DEGRADATION BUT NOT DISSOCIATION FROM NF-KAPPA-B [J].
MIYAMOTO, S ;
MAKI, M ;
SCHMITT, MJ ;
HATANAKA, M ;
VERMA, IM .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (26) :12740-12744