Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM)

被引：5911

作者：

Rust, Michael J.

Bates, Mark

Zhuang, Xiaowei

机构：

[1] Harvard Univ, Dept Phys, Cambridge, MA 02138 USA

[2] Harvard Univ, Div Engn & Appl Sci, Cambridge, MA 02138 USA

[3] Harvard Univ, Dept Chem & Chem Biol, Cambridge, MA 02138 USA

[4] Harvard Univ, Howard Hughes Med Inst, Cambridge, MA 02138 USA

来源：

NATURE METHODS | 2006年 / 3卷 / 10期

关键词：

D O I：

10.1038/nmeth929

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have developed a high-resolution fluorescence microscopy method based on high-accuracy localization of photoswitchable fluorophores. In each imaging cycle, only a fraction of the fluorophores were turned on, allowing their positions to be determined with nanometer accuracy. The fluorophore positions obtained from a series of imaging cycles were used to reconstruct the overall image. We demonstrated an imaging resolution of 20 nm. This technique can, in principle, reach molecular-scale resolution.

引用

收藏

页码：793 / 795

页数：3

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