Endothelial P-selectin as a target of heparin action in experimental melanoma lung metastasis

被引:169
作者
Ludwig, RJ
Boehme, B
Podda, M
Henschler, R
Jager, E
Tandi, C
Boehncke, WH
Zollner, TM
Kaufmann, R
Gille, J
机构
[1] Univ Frankfurt Klinikum, Zentrum Dermatol, Dept Dermatol, D-60590 Frankfurt, Germany
[2] Schering AG, RBA Dermatol, D-1000 Berlin, Germany
[3] Krankenhaus NW Frankfurt, Dept Hematol Oncol, Frankfurt, Germany
[4] German Red Cross Blood Ctr, Inst Transfus Med, Frankfurt, Germany
[5] Klin Forsch, Bad Nauheim, Germany
[6] Max Planck Inst Physiol & Clin Res, Dept Biol Mol, Bad Nauheim, Germany
关键词
D O I
10.1158/0008-5472.CAN-03-1054
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Spontaneous and experimental metastasis can be effectively inhibited by the widely used anticoagulant heparin in different tumor models. At the cellular level, many of the antimetastatic effects of heparin in vivo are due to its action on P-selectin-mediated binding. Whereas previous attention has focused on P-selectin-dependent tumor-cell-platelet interactions in blood-borne metastasis, we sought to address the potential contribution of endothelial P-selectin expression to adhesive events between the microvasculature and melanoma cells in vivo. Transplantation of bone marrow from P-selectin-deficient into wild-type mice conveyed inhibition of experimental melanoma metastasis. However, the extent to which bone marrow-conferred lack of platelet P-selectin expression attenuated melanoma lung metastasis was significantly less than that seen in P-selectin-deficient mice, suggesting that endothelial P-selectin expression may additionally contribute to formation of hematogenous metastases. This assumption was supported by our intravital microscopy studies, in which a significant proportion of melanoma cells were capable of directly interacting with postcapillary venules of the murine ear in a P-selectin-dependent manner. Heparin not only inhibits P-selectin-mediated melanoma cell rolling but also attenuates melanoma metastasis formation in vivo, further supporting the concept that endothelial P-selectin expression may represent an additional target of heparin action in experimental melanoma lung metastasis.
引用
收藏
页码:2743 / 2750
页数:8
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