Chemical structures of water-soluble polysaccharides from Rhizoma Panacis Japonici

被引：44

作者：

Huang, Zhiping ^{[1
,2
]}

Zhang, Lina ^{[1
]}

机构：

[1] Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China

[2] CASC, Acad 4, Inst 42, Xiangfan 441003, Peoples R China

来源：

CARBOHYDRATE RESEARCH | 2009年 / 344卷 / 09期

基金：

中国国家自然科学基金;

关键词：

Polysaccharides; Rhizoma Panacis Japonici; Water-soluble; Branch; alpha-(1 -> 4)-D-Glucan; MASS-SPECTROMETRY; GINSENG POLYSACCHARIDES; HELICOBACTER-PYLORI; CHAIN CONFORMATION; CHROMATOGRAPHY; INFECTION; NMR;

D O I：

10.1016/j.carres.2009.02.014

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Five polysaccharide samples, coded as RPS1, RPS2, RPS3, RPS4, and RPS5, were isolated stepwise from Rhizoma Panacis Japonici (RPJ) by using 0.15 M NaCl aqueous solution at 25 degrees C, boiling water at 120 degrees C, 0.5 M NaOH/0.01 M NaBH4 at 10 degrees C, 1.0 M NaOH/0.02 M NaBH4 at 10 degrees C, and 19 M HCOOH at 4 degrees C, respectively. The yields were 0.39%, 1.08%, 2.41%, 0.32%, and 0.04% for RPSI to RPS5, respectively. The chemical structures of the polysaccharides were highly branched alpha(-(1 -> 4)-D-glucan heteropolysaccharides and the values of degree of branch (DB) were in the range of 35-45% for RPS1 to RPS5. All of the polysaccharides were water soluble, and their solubility decreased from RPS1 to RPS5. The weight average molecular mass were 3.5 x 10(4), 1.47 x 10(5), 1.24 x 10(6), 9.26 x 10(5), and 1.36 x 10(6) for RPS1 to RPS5, respectively. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：1136 / 1140

页数：5

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