A sensitive and rapid method for mapping protein bound to DNA by atomic force microscopy

被引：2

作者：

Tanigawa, M

Machida, M

Okada, T

机构：

[1] NATL INST BIOSCI & HUMAN TECHNOL, DEPT MOL BIOL, TSUKUBA, IBARAKI 305, JAPAN

[2] JOINT RES CTR ATOM TECHNOL, TSUKUBA, IBARAKI 305, JAPAN

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1997年 / 61卷 / 10期

关键词：

DNA mapping; atomic force microscopy; Sp1; DNA-protein complex;

D O I：

10.1271/bbb.61.1751

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We addressed the strategies of mapping protein binding sites on a DNA fragment by atomic force microscopy (AFM). The protein binding site was uniquely mapped by distinguishing two termini of a linear DNA fragment. Our simple methods were found very useful to get information on transcription regulatory regions by taking advantage of long range and quick scanning by AFM.

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收藏

页码：1751 / 1753

页数：3

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