Nonchemical approach for reducing antinutritional factors in rapeseed (Brassica campestris var. Toria) and characterization of enzyme phytase

被引：20

作者：

Mahajan, A ^{[1
]}

Dua, S ^{[1
]}

机构：

[1] PANJAB UNIV, DEPT BOT, CHANDIGARH 160014, INDIA

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 1997年 / 45卷 / 07期

关键词：

antinutritional factors; characterization; germination; phytase;

D O I：

10.1021/jf9609136

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Antinutritional factors, viz, glucosinolates, phenols, and phytic acid, decreased with germination of rapeseed. Protein content declined during the first 24 h after imbibition and thereafter increased, Phytase activity increased with germination with a parallel decrease in phytic acid content. Two phytase fractions were separated during elution on DEAE-cellulose column, showing purifications of 26.75- and 19.96-fold, respectively. Optimum pH and temperature for both fractions were 5.2 and 50 degrees C, respectively. Enzyme stability decreased considerably after 50 degrees C, and phytase 2 was stable for a longer time. Mn, Co, Ca, and EDTA promoted the enzyme activity, whereas ions of Zn, Fe, Cu, fluoride, molybdate, and arsenate were inhibitory.

引用

页码：2504 / 2508

页数：5

共 27 条

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