Phage display identifies thioredoxin and superoxide dismutase as novel protein kinase C-interacting proteins: thioredoxin inhibits protein kinase C-mediated phosphorylation of histone

被引：48

作者：

Watson, JA

Rumsby, MG

Wolowacz, RG ^{[1
]}

机构：

[1] Univ York, Dept Biol, York YO1 5DD, N Yorkshire, England

[2] Smith & Nephew Plc, York YO1 5HN, N Yorkshire, England

来源：

BIOCHEMICAL JOURNAL | 1999年 / 343卷

关键词：

binding protein; cell signalling; overlay assay; oxidative stress; phagemid;

D O I：

10.1042/0264-6021:3430301

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Using phage display we identify the redox proteins thioredoxin and superoxide dismutase (SOD) as novel protein kinase C (PKC)-interacting proteins. Overlay assays demonstrated that PKC bound to immobilized thioredoxin, providing supporting evidence for the phage display results. Kinase assays demonstrated that SOD and thioredoxin were not direct substrates for PKC but that both proteins blocked autophosphorylation of PKC, Moreover, thioredoxin inhibited PKC-mediated phosphorylation of histone (IC50 of approx. 20 ng/ml).

引用

页码：301 / 305

页数：5

共 27 条

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