TGF-β activates genes identified by differential mRNA display in pancreatic rudiments

被引：3

作者：

Battelino, T

Miralles, F

Krzisnik, C

Scharfmann, R

Czernichow, P

机构：

[1] Univ Childrens Hosp, SI-1000 Ljubljana, Slovenia

[2] Hop Robert Debre, INSERM, U457, F-75019 Paris, France

来源：

PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY | 2000年 / 439卷 / 03期

关键词：

pancreatic development; TGF-beta; differential mRNA display; glucagon; B-carboxypeptidase;

D O I：

10.1007/s004240000078

中图分类号：

Q4 [生理学];

学科分类号：

071003 ;

摘要：

The effect of TGF-beta on gene activation in embryonic pancreatic rudiments was investigated using differential mRNA display. Several cDNA bands were augmented and some were suppressed in the presence of TGF-beta. Differentially expressed cDNAs were reamplified, sequenced, and sequences compared to the GeneBank database. Glucagone and brain alpha-tropomyosin cDNAs were identified from the group of augmented cDNAs, and B-carboxypeptidase form the group of suppressed cDNAs. PCR experiments were confirmed with Northern blots. Obtained results are in accordance with immunohistochemical findings and render differential mRNA display a useful technique in identifying differentially expressed genes in embryonic pancreatic rudiments. Several unknown differentially expressed cDNA sequences obtained in our experiments remain to be identified.

引用

页码：R26 / R28

页数：3

共 8 条

[1] Expression of neuronal traits in pancreatic beta cells - Implication of neuron-restrictive silencing factor/repressor element silencing transcription factor, a neuron-restrictive silencer [J].