Cloning and sequence of a thermostable multidomain xylanase from the bacterium Rhodothermus marinus

被引：48

作者：

Karlsson, EN

BartonekRoxa, E

Holst, O

机构：

[1] Department of Biotechnology, Ctr. for Chem. and Chem. Engineering, Lund University, S-221 00 Lund

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1997年 / 1353卷 / 02期

关键词：

xylanase; glycosyl hydrolase family 10; cellulose binding domain family IV; (Rhodothermus marinus);

D O I：

10.1016/S0167-4781(97)00093-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The gene (xyn1) encoding a Rhodothermus marinus xylanase has been cloned and expressed in Escherichia coli. The gene comprises 5 different domains in an unusual combination. The cellulose binding domains (CBDs) encoded by xyn1 are repeated in tandem at the N-terminus and show similarity with the CBD family IV. The xyn1-gene is the first example encoding a CBD family IV in combination with a xylan hydrolyzing catalytic domain of the glycosyl hydrolase family 10. (C) 1997 Elsevier Science B.V.

引用

页码：118 / 124

页数：7

共 28 条

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