Metabolites of conjugated isomers of linoleic acid (CLA) in the rat

被引：144

作者：

Sebedio, JL ^{[1
]}

Juaneda, P ^{[1
]}

Dobson, G ^{[1
]}

Ramilison, I ^{[1
]}

Martin, JD ^{[1
]}

Chardigny, JM ^{[1
]}

机构：

[1] SCOTTISH CROP RES INST,DUNDEE DD2 5DA,SCOTLAND

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-LIPIDS AND LIPID METABOLISM | 1997年 / 1345卷 / 01期

关键词：

conjugated isomer of linoleic acid; conjugated double bond; conversion; mass spectrum; (rat);

D O I：

10.1016/S0005-2760(97)00015-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Metabolism of conjugated isomers of linoleic acid (CLA) in rats was studied by feeding high quantities of CLA (180 mg/day) for six days to animals that had been reared on a fat-free diet for two weeks. After this period, animals were sacrificed and liver lipids extracted. High-performance liquid chromatography (HPLC) analyses with UV detection revealed the presence of conjugated polyunsaturated fatty acids in the total liver lipid methyl esters. After isolation by HPLC, three fatty acid metabolites were identified by gas liquid chromatography coupled with mass spectrometry as being C20:3 Delta 8,12,14, C20:4 Delta 5,8,12,14 and C20:4 Delta 5,8,11,13. A higher quantity of C20:4 Delta 5,8,12,14 compared to C20:4 Delta 5,8,11,13 was observed. These must arise from the elongation and desaturation of 18:2 Delta 10,12 and 18:2 Delta 9,11, respectively. (C) 1997 Elsevier Science B.V.

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页码：5 / 10

页数：6

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