Modeling dynamic light scattering of supercoiled DNA

被引：13

作者：

Klenin, K ^{[1
]}

Hammermann, M ^{[1
]}

Langowski, J ^{[1
]}

机构：

[1] German Canc Res Ctr, Div Biophys Macromol, D-69120 Heidelberg, Germany

来源：

MACROMOLECULES | 2000年 / 33卷 / 04期

关键词：

D O I：

10.1021/ma9914467

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

A new computational scheme, based on the Brownian dynamics and Monte Carlo techniques, was used to calculate the dynamic structure factor of supercoiled SV40 DNA (5243 base pairs) in 0.1 M sodium chloride solution. All model parameters are known independently, and none are fitted. The results are in good agreement with earlier dynamic light scattering measurements. The calculated dynamic structure factor, as a function of time, could be very well approximated by a simple analytical formula. The analysis of the experimental data based on this formula allows one to obtain the distribution function for relaxation times of intramolecular motions, if additional information about the DNA structure is available from Monte Carlo simulations. The range of relaxation times for supercoiled SV40 DNA comprises approximately 2 orders of magnitude, with the upper boundary exceeding 1 ms.

引用

页码：1459 / 1466

页数：8

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