Evaluation of GenoType and LiPA MYCOBACTERIA assays for identification of Finnish mycobacterial isolates

被引:41
作者
Mäkinen, J
Sarkola, A
Marjamäki, M
Viljanen, MK
Soini, H
机构
[1] Natl Publ Hlth Inst, Mycobacterial Reference Lab, Turku 20520, Finland
[2] Univ Turku, Turku Grad Sch Biomed Sci, Turku, Finland
[3] Univ Turku, Dept Med Microbiol, Turku, Finland
关键词
D O I
10.1128/JCM.40.9.3478-3481.2002
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Two DNA strip assays, INNO-LiPA MYCOBACTERIA and GenoType Mykobakterien, were evaluated for identification of 81 Finnish mycobacterial isolates. The LiPA assay correctly identified 89.4% of the 66 isolates studied, and the GenoType assay identified 95.1% of 81 isolates. The GenoType assay had a wider selection of species and less stringent temperature requirements.
引用
收藏
页码:3478 / 3481
页数:4
相关论文
共 25 条
[1]   RFLP of rRNA genes and sequencing of the 16S-23S rDNA intergenic spacer region of ammonia-oxidizing bacteria:: a phylogenetic approach [J].
Aakra, Å ;
Utåker, JB ;
Nes, IF .
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1999, 49 :123-130
[2]   EFFICIENT DISCRIMINATION OF MYCOBACTERIUM-TUBERCULOSIS STRAINS BY 16S-23S SPACER REGION-BASED RANDOM AMPLIFIED POLYMORPHIC DNA ANALYSIS [J].
ABED, Y ;
DAVINREGLI, A ;
BOLLET, C ;
DEMICCO, P .
JOURNAL OF CLINICAL MICROBIOLOGY, 1995, 33 (05) :1418-1420
[3]   IDENTIFICATION AND STRAIN DIFFERENTIATION OF MYCOBACTERIUM SPECIES ON THE BASIS OF DNA 16S-23S SPACER REGION POLYMORPHISM [J].
ABED, Y ;
BOLLET, C ;
DEMICCO, P .
RESEARCH IN MICROBIOLOGY, 1995, 146 (05) :405-413
[4]   Heterogeneity and clonality among isolates of Mycobacterium kansasii: Implications for epidemiological and pathogenicity studies [J].
Alcaide, F ;
Richter, I ;
Bernasconi, C ;
Springer, B ;
Hagenau, C ;
SchulzeRobbecke, R ;
Tortoli, E ;
Martin, R ;
Bottger, EC ;
Telenti, A .
JOURNAL OF CLINICAL MICROBIOLOGY, 1997, 35 (08) :1959-1964
[5]   Allele-specific PCR method based on pncA and oxyR sequences for distinguishing Mycobacterium bovis from Mycobacterium tuberculosis:: Intraspecific M-bovis pncA sequence polymorphism [J].
de los Monteros, LEE ;
Galán, JC ;
Gutiérrez, M ;
Samper, S ;
Marín, JFG ;
Martín, C ;
Domínguez, L ;
de Rafael, L ;
Baqúero, F ;
Gómez-Mampaso, E ;
Blázquez, J .
JOURNAL OF CLINICAL MICROBIOLOGY, 1998, 36 (01) :239-242
[6]   Epidemiology of infection by nontuberculous mycobacteria [J].
Falkinham, JO .
CLINICAL MICROBIOLOGY REVIEWS, 1996, 9 (02) :177-+
[7]   GENOTYPIC IDENTIFICATION OF MYCOBACTERIA BY NUCLEIC-ACID SEQUENCE DETERMINATION - REPORT OF A 2-YEAR EXPERIENCE IN A CLINICAL LABORATORY [J].
KIRSCHNER, P ;
SPRINGER, B ;
VOGEL, U ;
MEIER, A ;
WREDE, A ;
KIEKENBECK, M ;
BANGE, FC ;
BOTTGER, EC .
JOURNAL OF CLINICAL MICROBIOLOGY, 1993, 31 (11) :2882-2889
[8]  
Kirschner P, 1998, METH MOL B, V101, P349, DOI 10.1385/0-89603-471-2:349
[9]   Genetic diversity of Mycobacterium avium recovered from AIDS patients in the Caribbean as studied by a consensus IS1245-RFLP method and pulsed-field gel electrophoresis [J].
Legrand, E ;
Sola, C ;
Verdol, B ;
Rastogi, N .
RESEARCH IN MICROBIOLOGY, 2000, 151 (04) :271-283
[10]   Description of a novel Mycobacterium simiae allelic variant isolated from Caribbean AIDS patients by PCR-restriction enzyme analysis and sequencing of hsp65 gene [J].
Legrand, E ;
Goh, KS ;
Sola, C ;
Rastogi, N .
MOLECULAR AND CELLULAR PROBES, 2000, 14 (06) :355-363