Acyl and alkyl chain length of GPI-anchors is critical for raft association in vitro

被引：73

作者：

Benting, J

Rietveld, A

Ansorge, I

Simons, K

机构：

[1] European Mol Biol Lab, Cell Biol & Biophys Programme, D-69117 Heidelberg, Germany

[2] Max Planck Inst Mol Cell Biol & Genet, D-01307 Dresden, Germany

[3] Univ Heidelberg, Zentrum Mol Biol, D-69120 Heidelberg, Germany

来源：

FEBS LETTERS | 1999年 / 462卷 / 1-2期

关键词：

GPI-anchor; fatty acid; lipid raft;

D O I：

10.1016/S0014-5793(99)01501-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We determined the acyl and alkyl chain composition of GPI-anchors isolated from MDCK and Fischer rat thyroid (FRT) cells. Both cell lines synthesize GPI-anchors containing C16/C18 or C18/C18 saturated acyl and alkyl chains. The GPI-anchored placental alkaline phosphatase (PLAP) expressed in both cells is raft-associated and FLAP purified from FRT cells is raft-associated in vitro when reconstituted into liposomes containing raft lipids. In contrast, the GPI-anchored variant surface glycoprotein from Trypanosoma brucei which contains C14 acyl and alkyl chains shows no significant raft association after reconstitution in vitro. These data indicate that the acyl and alkyl chain composition of GPI-anchors determines raft association. (C) 1999 Federation of European Biochemical Societies.

引用

页码：47 / 50

页数：4

共 25 条

[1] On the origin of sphingolipid/cholesterol-rich detergent-insoluble cell membranes: Physiological concentrations of cholesterol and sphingolipid induce formation of a detergent-insoluble, liquid-ordered lipid phase in model membranes
Ahmed, SN
Brown, DA
London, E
[J]. BIOCHEMISTRY, 1997, 36 (36) : 10944 - 10953
[2] Transcription of 'inactive' expression sites in African trypanosomes leads to expression of multiple transferrin receptor RNAs in bloodstream forms
Ansorge, I
Steverding, D
Melville, S
Hartmann, C
Clayton, C
[J]. MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1999, 101 (1-2) : 81 - 94
[3] Association of GAP-43 with detergent-resistant membranes requires two palmitoylated cysteine residues
Arni, S
Keilbaugh, SA
Ostermeyer, AG
Brown, DA
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (43) : 28478 - 28485
[4] N-glycans mediate the apical sorting of a GPI-anchored, raft-associated protein in Madin-Darby canine kidney cells
Benting, JH
Rietveld, AG
Simons, K
[J]. JOURNAL OF CELL BIOLOGY, 1999, 146 (02) : 313 - 320
[5] BLIGH EG, 1959, CAN J BIOCHEM PHYS, V37, P911
[6] Functions of lipid rafts in biological membranes
Brown, DA
London, E
[J]. ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY, 1998, 14 : 111 - 136
[7] MECHANISM OF MEMBRANE ANCHORING AFFECTS POLARIZED EXPRESSION OF 2 PROTEINS IN MDCK CELLS
BROWN, DA
CRISE, B
ROSE, JK
[J]. SCIENCE, 1989, 245 (4925) : 1499 - 1501
[8] SORTING OF GPI-ANCHORED PROTEINS TO GLYCOLIPID-ENRICHED MEMBRANE SUBDOMAINS DURING TRANSPORT TO THE APICAL CELL-SURFACE
BROWN, DA
ROSE, JK
[J]. CELL, 1992, 68 (03) : 533 - 544
[9] GLYCOSYL-PHOSPHATIDYLINOSITOL MOIETY THAT ANCHORS TRYPANOSOMA-BRUCEI VARIANT SURFACE GLYCOPROTEIN TO THE MEMBRANE
FERGUSON, MAJ
HOMANS, SW
DWEK, RA
RADEMACHER, TW
[J]. SCIENCE, 1988, 239 (4841) : 753 - 759
[10] The dually acylated NH2-terminal domain of Gi1α is sufficient to target a green fluorescent protein reporter to caveolin-enriched plasma membrane domains -: Palmitoylation of caveolin-1 is required for the recognition of dually acylated G-protein α subunits in vivo
Galbiati, F
Volonté, D
Meani, D
Milligan, G
Lublin, DM
Lisanti, MP
Parenti, M
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (09) : 5843 - 5850

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