Nine novel angiotensin I-converting enzyme (ACE) inhibitory peptides from cuttlefish (Sepia officinalis) muscle protein hydrolysates and antihypertensive effect of the potent active peptide in spontaneously hypertensive rats

被引：160

作者：

Balti, Rafik ^{[1
]}

Bougatef, Ali ^{[2
]}

Sila, Assaad ^{[1
]}

Guillochon, Didier ^{[1
]}

Dhulster, Pascal ^{[1
]}

Nedjar-Arroume, Naima ^{[1
]}

机构：

[1] Univ Lille 1 Sci & Technol, UPRES EA 1026, Lab Proc Biol Genie Enzymat & Microbien ProBioGEM, PolytechLille IUT A, F-59655 Villeneuve Dascq, France

[2] Univ Sfax, Higher Inst Biotechnol Sfax, Sfax 3038, Tunisia

来源：

FOOD CHEMISTRY | 2015年 / 170卷

关键词：

ACE inhibitory peptide; Cuttlefish muscle protein; Enzymatic hydrolysate; Purification and identification; Antihypertensive effect; VAL-TYR; PURIFICATION; IDENTIFICATION; DIPEPTIDE; CASEIN;

D O I：

10.1016/j.foodchem.2013.03.091

中图分类号：

O69 [应用化学];

学科分类号：

070301 [无机化学];

摘要：

This study aimed to identify novel ACE inhibitory peptides from the muscle of cuttlefish. Proteins were hydrolyzed and the hydrolysates were then subjected to various types of chromatography to isolate the active peptides. Nine ACE inhibitory peptides were isolated and their molecular masses and amino acid sequences were determined using ESI-MS and ESI-MS/MS, respectively. The structures of the most potent peptides were identified as Val-Glu-Leu-Tyr-Pro, Ala-Phe-Val-Gly-Tyr-Val-Leu-Pro and Glu-Lys-Ser-Tyr-Glu-Leu-Pro. The first peptide displayed the highest ACE inhibitory activity with an IC50 of 5.22 mu M. Lineweaver-Burk plots suggest that Val-Glu-Leu-Tyr-Pro acts as a non-competitive inhibitor against ACE. Furthermore, antihypertensive effects in spontaneously hypertensive rats (SHR) also revealed that oral administration of Val-Glu-Leu-Tyr-Pro can decrease systolic blood pressure significantly (p < 0.01). These results suggest that the Val-Glu-Leu-Tyr-Pro would be a beneficial ingredient for nutraceuticals and pharmaceuticals acting against hypertension and its related diseases. Crown Copyright (C) 2013 Published by Elsevier Ltd. All rights reserved.

引用

页码：519 / 525

页数：7

共 30 条

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