Comparison of the performance of the NucliSENS EasyQ HPV E6/E7 mRNA assay and HPV DNA chip for testing squamous cell lesions of the. uterine cervix

被引:29
作者
Munkhdelger, Jijgee [1 ]
Choi, Yeonim [2 ,3 ]
Lee, Dongsup [1 ,4 ]
Kim, Sunghyun [2 ,5 ]
Kim, Geehyuk [2 ]
Park, Sangjung [2 ,6 ]
Choi, Eunhee [7 ]
Jin, Hyunwoo [8 ]
Jeon, Bo-Young [2 ]
Lee, Hyeyoung [2 ]
Park, Kwang Hwa [1 ]
机构
[1] Wonju Coll Med, Yonsei Univ, Dept Pathol, Wonju, South Korea
[2] Yonsei Univ, Coll Hlth Sci, Dept Biomed Lab Sci, 1 Yonseidae gil, Wonju 220710, South Korea
[3] Songho Coll, Dept Biomed Lab Sci, Hoengseoung, South Korea
[4] Hyejeon Coll, Dept Clin Lab Sci, Hongseong, South Korea
[5] Yonsei Univ, Inst Life Sci & Biotechnol, Seoul, South Korea
[6] Daegu Haany Univ, Coll Med Sci, Dept Clin Lab Sci, Daegu, South Korea
[7] Yonsei Univ, Wonju Coll Med, Inst Lifestyle Med, Wonju, South Korea
[8] Catholic Univ Pusan, Dept Clin Lab Sci, Coll Hlth Sci, Pusan, South Korea
关键词
Human papillomavirus; HPV E6/E7 mRNA assay; HPV DNA chips; High-grade cervical lesions; HUMAN-PAPILLOMAVIRUS TYPES; HIGH-RISK; INTRAEPITHELIAL NEOPLASIA; ONCOGENE TRANSCRIPTS; WOMEN; CANCER; PREVALENCE; PROOFER; E6; EXPRESSION;
D O I
10.1016/j.diagmicrobio.2014.04.004
中图分类号
R51 [传染病];
学科分类号
100201 [内科学];
摘要
This study aims to evaluate the clinical performance of the NucliSENS EasyQassay and compare it with HPV DNA genotyping for the detection of high-grade squamous intraepithelial lesions (HSIL) and cancer in a Korean population. In 188 total thin prep samples, the remaining fluid after cytology slide preparation was tested with Goodgene HPV DNA chips and the NucliSENS EasyQ HPV E6/E7 messenger RNA (mRNA) assay. The sensitivity and specificity of each test were calculated with HSIL and squamous cell carcinoma (SCC) as the disease endpoint. Out of the 188 samples, 139(74%) were positive for DNA of 14 HPV types, while 57(30%) cases were positive for E6/E7 mRNA. The DNA test was positive in cytology cases of SCC, HSIL, and atypical squamous cell. The mRNA test yielded results of 75%, 74%, 60%, 56%, and 29% positivity in abnormal cytology cases of SCC, HSIL, atypical squamous cells - cannot exclude HSIL, atypical squamous cells of undetermined significance, and low-grade squamous intraepithelial lesion, respectively. In normal cytology cases, the positivity rates were 9% and 53% for the mRNA and DNA tests, respectively. For detection of HSIL and SCC, the sensitivity of the mRNA test was 7436% and that of the DNA test was 100%, while the specificities of the tests were 85% and 40.83%, respectively. These findings suggest that the HPV E6/E7 mRNA assay can overcome the shortcoming of low specificity of DNA assays for clinical detection of high-grade cervical lesions and malignancies. (C) 2014 Elsevier Inc. All rights reserved.
引用
收藏
页码:422 / 427
页数:6
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