DNA fingerprint changes in tuberculosis: Reinfection, evolution, or laboratory error?

被引:41
作者
Glynn, JR
Yates, MD
Crampin, AC
Ngwira, BM
Mwaungulu, FD
Black, GF
Chaguluka, SD
Mwafulirwa, DT
Floyd, S
Murphy, C
Drobniewski, FA
Fine, PEM
机构
[1] Univ London London Sch Hyg & Trop Med, Infect Dis Epidemiol Unit, London WC1E 7HT, England
[2] Kings Coll Hosp Dulwich, HPA Mycobacterium Reference Unit, London, England
[3] Karonga Prevent Study, Chilumba, Malawi
基金
英国惠康基金;
关键词
D O I
10.1086/423144
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Background. DNA fingerprint patterns of Mycobacterium tuberculosis strains vary within individuals and between epidemiologically linked individuals because of pattern evolution, new infections, and laboratory error. We explored the importance of these factors. Methods. Cultures from individuals in northern Malawi who had been diagnosed with tuberculosis (TB) during 1996-2001 were fingerprinted with restriction fragment-length polymorphism (RFLP). Probable laboratory error was inferred by use of dates or isolated positive cultures. Pattern evolution was explored within and between individuals, and the relative importance of relapse and reinfection was estimated in individuals with recurrent TB. Results. RFLP results were available for 930 isolates from 806 individuals. The maximum laboratory-error rate was 3.9%. Pattern evolution was more common in linked individuals (17%) than on relapse (11%) or during treatment (3%). Twenty individuals had recurrent TB after completing treatment: in human immunodeficiency virus (HIV)-positive individuals, 7 of 12 recurrences were due to reinfection, compared with 0 of 8 in HIV-negative individuals (P=.01). Conclusions. The rate of DNA fingerprint-pattern evolution is not linear, and rates of change calculated from repeat cultures within individuals may not be applicable to transmission between individuals. The high proportion of recurrence due to reinfection found in HIV-positive individuals suggests that secondary prophylaxis and/or antiretroviral treatments are needed for such individuals.
引用
收藏
页码:1158 / 1166
页数:9
相关论文
共 35 条
[1]  
Bennett DE, 2002, EMERG INFECT DIS, V8, P1224
[2]   Retrospective detection of laboratory cross-contamination of Mycobacterium tuberculosis cultures with use of DNA fingerprint analysis [J].
Braden, CR ;
Templeton, GL ;
Stead, WW ;
Bates, JH ;
Cave, MD ;
Valway, SE .
CLINICAL INFECTIOUS DISEASES, 1997, 24 (01) :35-40
[3]   Interpretation of restriction fragment length polymorphism analysis of Mycobacterium tuberculosis isolates from a state with a large rural population [J].
Braden, CR ;
Templeton, GL ;
Cave, MD ;
Valway, S ;
Onorato, IM ;
Castro, KG ;
Moers, D ;
Yang, ZH ;
Stead, WW ;
Bates, JH .
JOURNAL OF INFECTIOUS DISEASES, 1997, 175 (06) :1446-1452
[4]   Simultaneous infection with multiple strains of Mycobacterium tuberculosis [J].
Braden, CR ;
Morlock, GP ;
Woodley, CL ;
Johnson, KR ;
Colombel, AC ;
Cave, MD ;
Yang, ZH ;
Valway, SE ;
Onorato, IM ;
Crawford, JT .
CLINICAL INFECTIOUS DISEASES, 2001, 33 (06) :E42-E47
[5]   DNA fingerprinting with two probes decreases clustering of Mycobacterium tuberculosis [J].
Burman, WJ ;
Reves, RR ;
Hawkes, AP ;
Rietmeijer, CA ;
Yang, ZH ;
ElHajj, H ;
Bates, JH ;
Cave, MD .
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 1997, 155 (03) :1140-1146
[6]   The incidence of false-positive cultures for Mycobacterium tuberculosis [J].
Burman, WJ ;
Stone, BL ;
Reves, RR ;
Wilson, ML ;
Yang, ZH ;
ElHajj, H ;
Bates, JH ;
Cave, MD .
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 1997, 155 (01) :321-326
[7]   STABILITY OF DNA FINGERPRINT PATTERN PRODUCED WITH IS6110 IN STRAINS OF MYCOBACTERIUM-TUBERCULOSIS [J].
CAVE, MD ;
EISENACH, KD ;
TEMPLETON, G ;
SALFINGER, M ;
MAZUREK, G ;
BATES, JH ;
CRAWFORD, JT .
JOURNAL OF CLINICAL MICROBIOLOGY, 1994, 32 (01) :262-266
[8]   Efficacy of secondary isoniazid preventive therapy among HIV-infected Southern Africans: time to change policy? [J].
Churchyard, GJ ;
Fielding, K ;
Charalambous, S ;
Day, JH ;
Corbett, EL ;
Hayes, RJ ;
Chaisson, RE ;
De Cock, KM ;
Samb, B ;
Grant, AD .
AIDS, 2003, 17 (14) :2063-2070
[9]  
Cohn DL, 1998, INT J TUBERC LUNG D, V2, P16
[10]  
Crampin AC, 2004, INT J TUBERC LUNG D, V8, P194