Refolding and reconstitution of human recombinant Bax inhibitor-1 into liposomes from inclusion bodies expressed in Escherichia coli

被引：4

作者：

Ahn, Taeho ^{[1
]}

Yun, Chul-Ho ^{[2
]}

Chae, Ho Zoon ^{[2
]}

Kim, Hyung-Ryong ^{[3
,4
]}

Chae, Han-Jung ^{[5
,6
]}

机构：

[1] Chonnam Natl Univ, Dept Biochem, Coll Vet Med, Kwangju 500757, South Korea

[2] Chonnam Natl Univ, Sch Biol Sci & Technol, Kwangju 500757, South Korea

[3] Wonkwang Univ, Sch Dent, Wonkwang Biomat Implant Res Inst, Iksan 570749, Chonbuk, South Korea

[4] Wonkwang Univ, Dept Dent Pharmacol, Iksan 570749, Chonbuk, South Korea

[5] Chonbuk Natl Univ, Sch Med, Inst Cardiovasc Res, Jeonju 561181, Chonbuk, South Korea

[6] Chonbuk Natl Univ, Dept Pharmacol, Jeonju 561181, Chonbuk, South Korea

来源：

PROTEIN EXPRESSION AND PURIFICATION | 2009年 / 66卷 / 01期

关键词：

Bax inhibitor-1; Liposomes; Refolding; Reconstitution; ENDOPLASMIC-RETICULUM; SUPPRESSOR; ANIMALS; PLANTS; YEAST;

D O I：

10.1016/j.pep.2009.01.005

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In this study, we report the simultaneous refolding and reconstitution of the recombinant Bax inhibitor-1 (BI-1) from inclusion bodies expressed in Escherichia coli. A functional assay showed that the resulting proteoliposomes responded to acidic conditions and triggered the release of entrapped Ca(2+) from liposomes. The secondary structure of the reconstituted BI-1 was also determined using circular dichroism, which revealed an increase of alpha-helix content and a decrease of random structure when exposed to acidic solutions. These conformational changes may be responsible for the proton ion-induced Ca(2+) release of BI-1. (C) 2009 Published by Elsevier Inc.

引用

页码：35 / 38

页数：4

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