3D Printing Surgical Implants at the clinic: A Experimental Study on Anterior Cruciate Ligament Reconstruction

被引：16

作者：

Liu, An ^{[1
,3
]}

Xue, Guang-huai ^{[2
,3
]}

Sun, Miao ^{[4
]}

Shao, Hui-feng ^{[2
,3
]}

Ma, Chi-yuan ^{[1
]}

Gao, Qing ^{[2
,3
]}

Gou, Zhong-ru ^{[5
]}

Yan, Shi-gui ^{[1
]}

Liu, Yan-ming ^{[4
]}

He, Yong ^{[2
,3
]}

机构：

[1] Zhejiang Univ, Sch Med, Affiliated Hosp 2, Dept Orthopaed Surg, Hangzhou 310009, Zhejiang, Peoples R China

[2] Zhejiang Univ, Coll Mech Engn, State Key Lab Fluid Power & Mechatron Syst, Hangzhou 310027, Zhejiang, Peoples R China

[3] Zhejiang Univ, Coll Mech Engn, Key Lab Printing Proc & Equipment Zhejiang Prov 3, Hangzhou 310027, Zhejiang, Peoples R China

[4] Zhejiang Univ, Sch Med, Affiliated Hosp 2, Dept Oral & Maxillofacial Surg, Hangzhou 310009, Zhejiang, Peoples R China

[5] Zhejiang Univ, Zhejiang California Int Nanosyst Inst, Hangzhou 310058, Zhejiang, Peoples R China

来源：

SCIENTIFIC REPORTS | 2016年 / 6卷

基金：

中国国家自然科学基金;

关键词：

MESENCHYMAL STEM-CELLS; CALCIUM-PHOSPHATE; IN-VIVO; SCAFFOLDS; TISSUES;

D O I：

10.1038/srep21704

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

Desktop three-dimensional (3D) printers (D3DPs) have become a popular tool for fabricating personalized consumer products, favored for low cost, easy operation, and other advantageous qualities. This study focused on the potential for using D3DPs to successfully, rapidly, and economically print customized implants at medical clinics. An experiment was conducted on a D3DP-printed anterior cruciate ligament surgical implant using a rabbit model. A well-defined, orthogonal, porous PLA screw-like scaffold was printed, then coated with hydroxyapatite (HA) to improve its osteoconductivity. As an internal fixation as well as an ideal cell delivery system, the osteogenic scaffold loaded with mesenchymal stem cells (MSCs) were evaluated through both in vitro and in vivo tests to observe bone-ligament healing via cell therapy. The MSCs suspended in Pluronic F-127 hydrogel on PLA/HA screw-like scaffold showed the highest cell proliferation and osteogenesis in vitro. In vivo assessment of rabbit anterior cruciate ligament models for 4 and 12 weeks showed that the PLA/HA screw-like scaffold loaded with MSCs suspended in Pluronic F-127 hydrogel exhibited significant bone ingrowth and bone-graft interface formation within the bone tunnel. Overall, the results of this study demonstrate that fabricating surgical implants at the clinic (fab@clinic) with D3DPs can be feasible, effective, and economical.

引用

页数：13

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