Development of a hypoxanthine biosensor based on immobilized xanthine oxidase chemically modified electrode

被引:36
作者
Hu, SS [1 ]
Liu, CC [1 ]
机构
[1] CASE WESTERN RESERVE UNIV, EDISON SENSOR TECHNOL CTR, CLEVELAND, OH 44106 USA
关键词
biosensor; enzyme electrode; modified electrode; amperometry; hypoxanthine; xanthine oxidase; fish;
D O I
10.1002/elan.1140090504
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
An amperometric biosensor for hypoxanthine determination has been developed The sensor uses a Nafion-paraquat chemically modified glassy-carbon electrode. It detects the oxygen consumed by the enzymatic reaction catalyzed by xanthine oxidase which is immobilized on the electrode surface. The sensor responds linearly to hypoxanthine over the concentration range of 1 x 10(-6) M - 2 x 10(-4) M. The biosensor call be reused for more than 100 times without significant deterioration in performance. After 32 days storage at 3-5 degrees C, the sensor response remains at 68 % of the initial level. The high sensitivity, selectivity and stability of this biosensor demonstrates its practical applicability for a simple, rapid and economical determination of hypoxanthine in fish samples.
引用
收藏
页码:372 / 377
页数:6
相关论文
共 38 条
[1]   RECOMMENDED GENERAL-METHODS FOR THE EXAMINATION OF FISH AND FISH PRODUCTS [J].
不详 .
ANALYST, 1979, 104 (1238) :434-450
[2]   ROTATING-RING-DISC ANALYSIS OF IRON TETRA(N-METHYLPYRIDYL)PORPHYRIN IN ELECTROCATALYSIS OF OXYGEN [J].
BETTELHEIM, A ;
KUWANA, T .
ANALYTICAL CHEMISTRY, 1979, 51 (13) :2257-2260
[3]  
Burt J. R., 1968, Journal of Food Technology, V3, P165, DOI 10.1361/152981503770351274
[4]  
BURT JR, 1977, PROCESS BIOCHEM, V12, P32
[5]   GAMMA-IRRADIATED POLYMER-MODIFIED GRAPHITE-ELECTRODES WITH ENHANCED RESPONSE TO CATECHOL [J].
COURY, LA ;
BIRCH, EM ;
HEINEMAN, WR .
ANALYTICAL CHEMISTRY, 1988, 60 (06) :553-560
[6]   FLOW-INJECTION DETERMINATION OF NITRITE BY AMPEROMETRIC DETECTION AT A MODIFIED ELECTRODE [J].
COX, JA ;
KULKARNI, KR .
ANALYST, 1986, 111 (10) :1219-1220
[7]   CATALYSIS OF DIOXYGEN REDUCTION AT GLASSY-CARBON AND CARBON-FIBER ELECTRODES COATED WITH POLY-COBALT(O-AMINOPHENYL)PORPHYRIN FILM [J].
DONG, SJ ;
KUWANA, T .
ELECTROCHIMICA ACTA, 1988, 33 (05) :667-674
[8]   AMPEROMETRIC SENSOR FOR HYPOXANTHINE AND XANTHINE BASED ON THE DETECTION OF URIC-ACID [J].
GONZALEZ, E ;
PARIENTE, F ;
LORENZO, E ;
HERNANDEZ, L .
ANALYTICA CHIMICA ACTA, 1991, 242 (02) :267-273
[9]  
GUILBAULT GG, 1984, ANAL USES IMMOBILIZE
[10]   THE DETERMINATION OF PURINES IN FRESH AND SEA-WATER BY CATHODIC STRIPPING VOLTAMMETRY AFTER COMPLEXATION WITH COPPER(I) [J].
HOUSEHAM, BC ;
VAN DEN BERG, CMG ;
RILEY, JP .
ANALYTICA CHIMICA ACTA, 1987, 200 (01) :291-303