Protective effect of dietary supplementation with L-arginine and L-carnitine on hypoxia/reoxygenation-induced necrotizing enterocolitis in young mice

被引:36
作者
Akisu, M
Ozmen, D
Baka, M
Habif, S
Yalaz, M
Arslanoglu, S
Kultursay, N
Bayindir, O
机构
[1] Ege Univ, Sch Med, Dept Pediat, Izmir, Turkey
[2] Ege Univ, Sch Med, Dept Clin Biochem, Izmir, Turkey
[3] Ege Univ, Sch Med, Dept Histol, Izmir, Turkey
来源
BIOLOGY OF THE NEONATE | 2002年 / 81卷 / 04期
关键词
L-arginine; L-carnitine; nitric oxide; necrotizing enterocolitis;
D O I
10.1159/000056757
中图分类号
R72 [儿科学];
学科分类号
100202 ;
摘要
Oxygen-derived free radicals are important components of gastrointestinal injury in necrotizing enterocolitis (NEC). In the present investigation, we examined the protective actions of L-arginine, a nitric oxide synthase substrate, and L-carnitine against hypoxia-reoxygenation (H/R) induced NEC in young mice. Young mice were divided into four groups: group 1 mice were subjected to H/R only; group 2 H/R mice were supplemented with L-arginine in the drinking water (2 g/I) for 7 days; group 3 H/R mice were given L-carnitine solution in water (50 mg/ kg p.o.) for 7 days, and group 4 mice served as controls. Hypoxia was induced by placing the mice in a 100% CO2 chamber for 5 min. After hypoxia, the mice were reoxygenated for 10 min with 100% oxygen. We examined the intestinal lesions by light microscopy and measured the intestinal generation of thiobarbituric acid reactive substances (TBARS) and the activities of superoxide dismutase and catalase in the H/R-induced model of NEC. In both L-arginine and L-carnitine groups, the NEC-induced intestinal tissue damage was greatly attenuated, with necrosis limited partially to the mucosa. The tissue TBARS level was significantly higher in group 1 than in any of the other groups (p < 0.001). However, those treated with L-arginine and L-carnitine had TBARS levels similar to those in the control animals. An increased tissue concentration of nitrate, a stable metabolite of nitric oxide, was found in the L-arginine-supplemented group as compared with the control group (p < 0.05). Both superoxide dismutase and catalase activities in the intestine were similar in H/R groups when compared with the intestine of control animals. The present study suggests that oxygen-derived free radicals are involved in the pathogenesis of H/R-induced NEC. This study also shows that dietary supplementation with L-arginine and L-carnitine ameliorates the histological evidence of H/R-induced intestinal injury and significantly decreases lipid peroxidation in H/R-induced bowel injury. Based on these findings, the beneficial effects of L-arginine and L-carnitine in this model may be mediated via mechanisms preventing free radical damage. Copyright (C) 2002 S. Karger AG, Basel.
引用
收藏
页码:260 / 265
页数:6
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