Changes in laser-induced fluorescence responses of 3T3 fibroblasts to repetitive thermal stress

被引：8

作者：

Beuthan, J. ^{[1
]}

Dressler, C. ^{[2
]}

Zabarylo, U. ^{[1
]}

Minet, O. ^{[1
]}

机构：

[1] Charite Univ Med Berlin, D-14195 Berlin, Germany

[2] Laser & Med Technol GmbH, D-14195 Berlin, Germany

来源：

LASER PHYSICS LETTERS | 2009年 / 6卷 / 04期

关键词：

laser-induced fluorescence; thermal stress; systems biology; SCANNING MICROSCOPY; SPECTROSCOPY; BARRIER;

D O I：

10.1002/lapl.200810134

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

The combined experimental use of laser-induced autofluorescence of cellular metabolites and methodological fundamentals of systems biology will provide access to biological thermal stress analysis on a sub cellular level. A test setup incorporating a pulsed nitrogen laser was realized with which autofluorescence of the coenzyme NADH could be measured in living 3T3 cells. The cells were subjected to different temperature stress at repetitive time intervals. When subjected to a simple mathematical analysis, the NADH concentration change measured through autofluorescence in biological cells exhibited approximate concentration-equivalent balance curves. These results add up to the fundamental know-how about the dosimetry of thermally therapeutic methods.

引用

页码：317 / 320

页数：4

共 20 条

[1] Study of vitamin A distribution in rats by laser induced fluorescence spectroscopy [J].