Visualization of molecular interactions by fluorescence complementation

被引：321

作者：

Kerppola, Tom K. ^{[1
]}

机构：

[1] Univ Michigan, Sch Med, Howard Hughes Med Inst, Ann Arbor, MI 48109 USA

[2] Univ Michigan, Sch Med, Dept Biol Chem, Ann Arbor, MI 48109 USA

来源：

NATURE REVIEWS MOLECULAR CELL BIOLOGY | 2006年 / 7卷 / 06期

关键词：

D O I：

10.1038/nrm1929

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The visualization of protein complexes in living cells enables the examination of protein interactions in their normal environment and the determination of their subcellular localization. The bimolecular fluorescence complementation assay has been used to visualize interactions among multiple proteins in many cell types and organisms. Modified forms of this assay have been used to visualize the competition between alternative interaction partners and the covalent modification of proteins by ubiquitin-family peptides.

引用

页码：449 / 456

页数：8

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