Deglycosylation of Glucose Oxidase for Direct and Efficient Glucose Electrooxidation on a Glassy Carbon Electrode

被引：105

作者：

Courjean, Olivier ^{[1
]}

Gao, Feng ^{[1
]}

Mano, Nicolas ^{[1
]}

机构：

[1] Univ Bordeaux, Ctr Rech Paul Pascal, UPR 8641, F-33600 Pessac, France

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2009年 / 48卷 / 32期

关键词：

biosensors; direct electron transfer; electrochemistry; enzymes; glucose oxidases; ASPERGILLUS-NIGER; ANGSTROM RESOLUTION; REDOX ENZYMES;

D O I：

10.1002/anie.200902191

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Down to the bare bones: When a glucose oxidase (GOx, left) was deglycosylated, the modified enzyme (right) immobilized on a vitreous carbon electrode came into closer electrical contact with the surface than the native enzyme. Glucose was electrooxidized directly on a monolayer of deglycosylated GOx on a carbon electrode at the unprecedented reducing potential of -200 mV versus Ag/AgCl and with a current density of 235 μA cm-2. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：5897 / 5899

页数：3

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