Quantitative vitreous collection using a modified infusion fluid during vitrectomy

Purpose: To develop a vitreous sampling method that increases yield while reducing risk of harm to the patient. Methods: Potential negative effects of fluoresceinated BSS(R) infusion solution on biologic activity were assessed by monitoring changes in fibroblast contraction of collagen gels in response to serum or vitreous. Paired vitreous samples were collected from ten patients before and during infusion of fluoresceinated BSS(R) solution. The extent to which the vitreous was diluted was calculated by comparing the levels of fluorescence in the samples with that in fluoresceinated BSS(R). Protein concentrations and levels of contraction-stimulating activity were measured for each sample. Results: Fluoresceinated BSS(R) did not alter fibroblast morphology, rate, or extent of gel contraction. Differences in corrected protein concentrations and biologic activities of the undiluted and diluted homogenous vitreous samples were not statistically significant. Conclusions: The use of fluoresceinated BSS(R) infusion yields larger vitreous samples from which the native biochemical characteristics can be determined. Patient safety during collection is enhanced because ocular hypotony and collapse can be avoided.