Interaction of Ras with phosphoinositide 3-kinase gamma

被引：77

作者：

Rubio, I

RodriguezViciana, P

Downward, J

Wetzker, R

机构：

[1] MAX PLANCK RES UNIT MOL CELL BIOL,D-07747 JENA,GERMANY

[2] IMPERIAL CANC RES FUND,SIGNAL TRANSDUCT LAB,LONDON WC2A 3PX,ENGLAND

来源：

BIOCHEMICAL JOURNAL | 1997年 / 326卷

关键词：

D O I：

10.1042/bj3260891

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Phosphoinositide 3-kinase gamma (PI3K gamma) can be activated in vitro by both alpha and beta gamma subunits of heterotrimeric G-proteins and does not interact with p85, the regulatory subunit of PI3K alpha. Here we demonstrate the binding of Ras to PI3K gamma in vitro. An N-terminal region of PI3K gamma was identified as a binding site for Ras. After co-expression with PI3K gamma in COS-7 cells, Ras induced only a modest increase in PI3K activity compared with the stimulation of PI3K alpha by Ras in the same cells.

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页码：891 / 895

页数：5

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