Identification of insulin variants using Raman spectroscopy

被引：66

作者：

Ortiz, C

Zhang, DM

Xie, Y

Davisson, VJ

Ben-Amotz, D ^{[1
]}

机构：

[1] Purdue Univ, Dept Chem, W Lafayette, IN 47907 USA

[2] Purdue Univ, Dept Med Chem & Mol Pharmacol, W Lafayette, IN 47907 USA

来源：

ANALYTICAL BIOCHEMISTRY | 2004年 / 332卷 / 02期

关键词：

insulin; variants; Raman; classification;

D O I：

10.1016/j.ab.2004.06.013

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Drop coating deposition Raman (DCDR) spectroscopy is used to obtain high-quality normal Raman spectra from small volumes (10mul) of dilute insulin solutions (3-400muM) for spectral identification and chromatographic detection. The results are used to demonstrate the spectroscopic classification (identification) of three natural insulin variants-human, bovine, and porcine-that differ by between one and three amino acid residues. DCDR measurements were performed on solutions obtained from reverse phase high-performance liquid chromatography (RP-HPLC) eluent fractions, either before or after lyophilization. Classification is demonstrated using replicate DCDR measurements, followed by normalized Savitsky-Golay second derivative preprocessing and partial least squares training with either leave-one-out or batch-to-batch testing. (C) 2004 Elsevier Inc. All rights reserved.

引用

页码：245 / 252

页数：8

共 33 条

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